FN Archimer Export Format
PT J
TI Identification, Characterization, and Diel Pattern of Expression of Canonical Clock Genes in Nephrops norvegicus (Crustacea: Decapoda) Eyestalk
BT 
AF SBRAGAGLIA, Valerio
   LAMANNA, Francesco
   MAT, Audrey
   ROTLLANT, Guiomar
   JOLY, Silvia
   KETMAIER, Valerio
   DE LA IGLESIA, Horacio O.
   AGUZZI, Jacopo
AS 1:1;2:2;3:3;4:1;5:1;6:4;7:3;8:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;
C1 ICM CSIC, Inst Marine Sci, Barcelona, Spain.
   Univ Potsdam, Inst Biochem & Biol, Unit Evolutionary Biol Systemat Zool, Potsdam, Germany.
   Univ Washington, Dept Biol, Seattle, WA 98195 USA.
   Univ Roma La Sapienza, Dept Biol & Biotechnol Charles Darwin, I-00185 Rome, Italy.
C2 ICM CSIC, SPAIN
   UNIV POTSDAM, GERMANY
   UNIV WASHINGTON, USA
   UNIV ROMA LA SAPIENZA, ITALY
IF 3.057
TC 24
UR https://archimer.ifremer.fr/doc/00483/59476/62314.pdf
   https://archimer.ifremer.fr/doc/00483/59476/62315.pdf
   https://archimer.ifremer.fr/doc/00483/59476/62316.pdf
   https://archimer.ifremer.fr/doc/00483/59476/62317.zip
LA English
DT Article
AB The Norway lobster, Nephrops norvegicus, is a burrowing decapod with a rhythmic burrow emergence (24 h) governed by the circadian system. It is an important resource for European fisheries and its behavior deeply affects its availability. The current knowledge of Nephrops circadian biology is phenomenological as it is currently the case for almost all crustaceans. In attempt to elucidate the putative molecular mechanisms underlying circadian gene regulation in Nephrops, we used a transcriptomics approach on cDNA extracted from the eyestalk, a structure playing a crucial role in controlling behavior of decapods. We studied 14 male lobsters under 12-12 light-darkness blue light cycle. We used the Hiseq 2000 Illumina platform to sequence two eyestalk libraries (under light and darkness conditions) obtaining about 90 millions 100-bp paired-end reads. Trinity was used for the de novo reconstruction of transcriptomes; the size at which half of all assembled bases reside in contigs (N50) was equal to 1796 (light) and 2055 (darkness). We found a list of candidate clock genes and focused our attention on canonical ones: timeless, period, clock and bmal1. The cloning of assembled fragments validated Trinity outputs. The putative Nephrops clock genes showed high levels of identity (blastx on NCBI) with known crustacean clock gene homologs such as Eurydice pulchra (period: 47%, timeless: 59%, bmal1: 79%) and Macrobrachium rosenbergii (clock: 100%). We also found a vertebrate-like cryptochrome 2. RT-qPCR showed that only timeless had a robust diel pattern of expression. Our data are in accordance with the current knowledge of the crustacean circadian clock, reinforcing the idea that the molecular clockwork of this group shows some differences with the established model in Drosophila melanogaster. 
PY 2015
PD NOV
SO Plos One
SN 1932-6203
PU Public Library Science
VL 10
IS 11
UT 000364029800028
DI 10.1371/journal.pone.0141893
ID 59476
ER

EF