FN Archimer Export Format
PT J
TI Ostreopsis lenticularis Y. Fukuyo (Dinophyceae, Gonyaulacales) from French Polynesia (South Pacific Ocean): A revisit of its morphology, molecular phylogeny and toxicity
BT 
AF Chomérat, Nicolas
   Bilien, Gwenael
   DERRIEN, Amelie
   Henry, Kévin
   Ung, André
   Viallon, Jérôme
   Darius, Hélène Taiana
   Mahana iti Gatti, Clémence
   Roué, Mélanie
   Hervé, Fabienne
   Réveillon, Damien
   Amzil, Zouher
   Chinain, Mireille
AS 1:1;2:1;3:1;4:2;5:2;6:2;7:2;8:2;9:3;10:4;11:4;12:4;13:2;
FF 1:PDG-ODE-LITTORAL-LERBO;2:PDG-ODE-LITTORAL-LERBO;3:PDG-ODE-LITTORAL-LERBO;4:;5:;6:;7:;8:;9:;10:PDG-ODE-DYNECO-PHYC;11:PDG-ODE-DYNECO-PHYC;12:PDG-ODE-DYNECO-PHYC;13:;
C1 Ifremer, LER BO, Station of Marine Biology of Concarneau, Place de la Croix, F-29900, Concarneau, France
   Institut Louis Malardé, Laboratoire des Micro-algues toxiques, UMR 241-EIO, PO box 30, 98713, Papeete, Tahiti, French Polynesia
   Institut de Recherche pour le Développement (IRD), UMR 241-EIO, PO box 529, 98713, Papeete, Tahiti, French Polynesia
   Ifremer, Phycotoxins Laboratory, BP 21105, F-44311, Nantes Cedex 3, France
C2 IFREMER, FRANCE
   INST LOUIS MALARDE, FRANCE
   IRD, FRANCE
   IFREMER, FRANCE
SI CONCARNEAU
   NANTES
SE PDG-ODE-LITTORAL-LERBO
   PDG-ODE-DYNECO-PHYC
UM EIO
IN WOS Ifremer UPR
   WOS Cotutelle UMR
   copubli-france
   copubli-p187
IF 3.707
TC 27
UR https://archimer.ifremer.fr/doc/00486/59747/62887.pdf
LA English
DT Article
DE ;ITS-5.8S rDNA;LSU rDNA;Microscopy;Ostreopsis lenticularis;Taxonomy;Toxins
AB To date, the genus Ostreopsis comprises eleven described species, of which seven are toxigenic and produce various compounds presenting a major threat to human and environmental health. The taxonomy of several of these species however remains controversial, as it was based mostly on morphological descriptions leading, in some cases, to ambiguous interpretations and even possible misidentifications. The species Ostreopsis lenticularis was first described by Y. Fukuyo from French Polynesia using light microscopy observations, but without genetic information associated. The present study aims at revisiting the morphology, molecular phylogeny and toxicity of O. lenticularis based on the analysis of 47 strains isolated from 4 distinct locales of French Polynesia, namely the Society, Australes, Marquesas and Gambier archipelagos. Observations in light, epifluorescence and field emission scanning electron microscopy of several of these strains analyzed revealed morphological features in perfect agreement with the original description of O. lenticularis. Cells were oval, not undulated, 60.5–94.4 μm in dorso-ventral length, 56.1–78.2 μm in width, and possessed a typical plate pattern with thecal plates showing two sizes of pores. Phylogenetic analyses inferred from the LSU rDNA and ITS–5.8S sequences revealed that the 47 strains correspond to a single genotype, clustering with a strong support with sequences previously ascribed to Ostreopsis sp. 5. Clonal cultures of O. lenticularis were also established and further tested for their toxicity using the neuroblastoma cell-based assay and LCMS/MS analyses. None of the 19 strains tested showed toxic activity on neuroblastoma cells, while LCMS/MS analyses performed on the strains from Tahiti Island (i.e. type locality) confirmed that palytoxin and related structural analogs were below the detection limit. These findings allow to clarify unambiguously the genetic identity of O. lenticularis while confirming previous results from the Western Pacific which indicate that this species shows no toxicity, thus stressing the need to reconsider its current classification within the group of toxic species. 
PY 2019
PD APR
SO Harmful Algae
SN 1568-9883
PU Elsevier BV
VL 84
UT 000471087800011
BP 95
EP 111
DI 10.1016/j.hal.2019.02.004
ID 59747
ER

EF