TY - JOUR T1 - Optimal control of bacterial growth for the maximization of metabolite production A1 - Yegorov,Ivan A1 - Mairet,Francis A1 - de Jong,Hidde A1 - Gouze,Jean-Luc AD - North Dakota State Univ, Fargo, ND 58105 USA. AD - IFREMER, PBA, Nantes, France. AD - Univ Grenoble Alpes, INRIA, F-38000 Grenoble, France. AD - Univ Cote dAzur, INRIA, INRA,CNRS, BIOCORE Team,Inria Sophia Antipolis Mediterranee, 2004 Route Lucioles, F-06902 Valbonne, France. UR - https://doi.org/10.1007/s00285-018-1299-6 DO - 10.1007/s00285-018-1299-6 KW - Optimal control KW - Nonlinear dynamical systems KW - Mathematical modelling KW - Bacterial growth KW - Biotechnology N2 - Microorganisms have evolved complex strategies for controlling the distribution of available resources over cellular functions. Biotechnology aims at interfering with these strategies, so as to optimize the production of metabolites and other compounds of interest, by (re)engineering the underlying regulatory networks of the cell. The resulting reallocation of resources can be described by simple so-called self-replicator models and the maximization of the synthesis of a product of interest formulated as a dynamic optimal control problem. Motivated by recent experimental work, we are specifically interested in the maximization of metabolite production in cases where growth can be switched off through an external control signal. We study various optimal control problems for the corresponding self-replicator models by means of a combination of analytical and computational techniques. We show that the optimal solutions for biomass maximization and product maximization are very similar in the case of unlimited nutrient supply, but diverge when nutrients are limited. Moreover, external growth control overrides natural feedback growth control and leads to an optimal scheme consisting of a first phase of growth maximization followed by a second phase of product maximization. This two-phase scheme agrees with strategies that have been proposed in metabolic engineering. More generally, our work shows the potential of optimal control theory for better understanding and improving biotechnological production processes. Y1 - 2019/03 PB - Springer Heidelberg JF - Journal Of Mathematical Biology SN - 0303-6812 VL - 78 IS - 4 SP - 985 EP - 1032 ID - 59791 ER -