FN Archimer Export Format
PT J
TI Response of a kleptoplastidic foraminifer to heterotrophic starvation: photosynthesis and lipid droplet biogenesis
BT 
AF Jauffrais, Thierry
   LeKieffre, Charlotte
   Schweizer, Magali
   Jesus, Bruno
   Metzger, Edouard
   Geslin, Emmanuelle
AS 1:1,2;2:1;3:1;4:3;5:1;6:1;
FF 1:PDG-RBE-LEADNC;2:;3:;4:;5:;6:;
C1 UMR CNRS 6112 LPG-BIAF, Bio-Indicateurs Actuels et Fossiles, Université d'Angers, 2 Boulevard Lavoisier, 49045 Angers CEDEX 1, France
   Ifremer, RBE/LEAD, 101 Promenade Roger Laroque, 98897 Nouméa, New Caledonia
   EA2160, Laboratoire Mer Molécules Santé, Université de Nantes, Nantes, France
C2 UNIV ANGERS, FRANCE
   IFREMER, FRANCE
   UNIV NANTES, FRANCE
SI NOUMEA
SE PDG-RBE-LEADNC
IN WOS Ifremer UPR
   copubli-france
   copubli-univ-france
IF 3.675
TC 10
UR https://archimer.ifremer.fr/doc/00487/59894/63074.pdf
LA English
DT Article
DE ;foraminifera;electron microscopy;DNA barcoding;kleptoplasty;lipids
AB The aim of this work is to document the complex nutritional strategy developed by kleptoplastic intertidal foraminifera. We study the mixotrophic ability of a common intertidal foraminifer, E. williamsoni, by (1) investigating the phylogenetic identity of the foraminiferal kleptoplasts, (2) following their oxygenic photosynthetic capacity, and (3) observing the modification in cellular ultrastructural features in response to photoautotrophic conditions. This was achieved by coupling molecular phylogenetic analyses and TEM observations with non-destructive measurements of kleptoplast O2 production over a 15-day experimental study. Results show that the studied E. williamsoni actively selected kleptoplasts mainly from pennate diatoms and had the ability to produce oxygen, up to 13.4 nmol O2 cell−1 d−1, from low to relatively high irradiance over at least 15 days. Ultrastructural features and photophysiological data showed significant differences over time, the number of lipid droplets, residual bodies and the dark respiration increased; whereas, the number of kleptoplasts decreased accompanied by a minor decrease of the photosynthetic rate. These observations suggest that in E. williamsoni kleptoplasts might provide extra carbon storage through lipid droplets synthesis and highlight the complexity of E. williamsoni feeding strategy and the necessity of further dedicated studies regarding mechanisms developed by kleptoplastidic foraminifera for carbon partitioning and storage. 
PY 2019
PD MAY
SO Fems Microbiology Ecology
SN 0168-6496
PU Oxford University Press (OUP)
VL 95
IS 5
UT 000475971000007
DI 10.1093/femsec/fiz046
ID 59894
ER

EF