FN Archimer Export Format
PT J
TI A study of autophagy in hemocytes of the Pacific oyster,   Crassostrea gigas
BT 
AF Picot, Sandy
   Morga, Benjamin
   Faury, Nicole
   Chollet, Bruno
   Dégremont, Lionel
   Travers, Marie-Agnes
   Renault, Tristan
   Arzul, Isabelle
AS 1:1;2:1;3:1;4:1;5:1;6:1;7:2;8:1;
FF 1:PDG-RBE-SGMM-LGPMM;2:PDG-RBE-SGMM-LGPMM;3:PDG-RBE-SGMM-LGPMM;4:PDG-RBE-SGMM-LGPMM;5:PDG-RBE-SGMM-LGPMM;6:PDG-RBE-SGMM-LGPMM;7:PDG-RBE;8:PDG-RBE-SGMM-LGPMM;
C1 SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, Ifremer, La Tremblade, France
   Département Ressources Biologiques et Environnement, Ifremer, Nantes, France
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI LA TREMBLADE
   NANTES
SE PDG-RBE-SGMM-LGPMM
   PDG-RBE
IN WOS Ifremer UPR
IF 9.77
TC 29
UR https://archimer.ifremer.fr/doc/00487/59905/63087.pdf
LA English
DT Article
DE ;Autophagosome;autophagy;Crassostrea gigas;flow cytometry;fluorescence microscopy;hemocytes;transmission electron microscopy
AB Macroautophagy is a mechanism that is involved in various cellular processes, including cellular homeostasis and innate immunity. This pathway has been described in organisms ranging in complexity from yeasts to mammals, and recent results indicate that it occurs in the mantle of the Pacific oyster, Crassostrea gigas. However, the autophagy pathway has never been explored in the hemocytes of C. gigas, which are the main effectors of its immune system and thus play a key role in the defence of the Pacific oyster against pathogens. To investigate autophagy in oyster hemocytes, tools currently used to monitor this mechanism in mammals, including flow cytometry, fluorescent microscopy and transmission electron microscopy, were adapted and applied to the hemocytes of the Pacific oyster. Oysters were exposed for 24 and 48 h to either an autophagy inducer (carbamazepine, which increases the production of autophagosomes) or an autophagy inhibitor (ammonium chloride, which prevents the degradation of autophagosomes). Autophagy was monitored in fresh hemocytes withdrawn from the adductor muscles of oysters using a combination of the three aforementioned methods. We successfully labelled autophagosomes and observed them by flow cytometry and fluorescence microscopy, and then used electron microscopy to observe ultrastructural modifications related to autophagy, including the presence of double-membrane-bound vacuoles. Our results demonstrated that autophagy occurs in hemocytes of C. gigas and can be modulated by molecules known to modulate autophagy in other organisms. This study describes an integrated approach that can be applied to investigate autophagy in marine bivalves at the cellular level. 
PY 2019
PD OCT
SO Autophagy
SN 1554-8627
PU Informa UK Limited
VL 15
IS 10
UT 000482334100007
BP 1801
EP 1809
DI 10.1080/15548627.2019.1596490
ID 59905
ER

EF