FN Archimer Export Format
PT J
TI Abundance and diversity of Planctomycetes in a Tyrrhenian coastal system of central Italy
BT 
AF PIZZETTI, Ilaria
   GOBET, Angelique
   FUCHS, Bernhard M.
   AMANN, Rudolf
   FAZI, Stefano
AS 1:1,2;2:3,4;3:2;4:2;5:1;
FF 1:;2:;3:;4:;5:;
C1 Water Res Inst IRSA CNR, I-00015 Rome, Italy.
   Max Planck Inst Marine Microbiol, D-28359 Bremen, Germany.
   CNRS, UMR 7144, Stn Biol Roscoff, F-29680 Roscoff, France.
   Univ Paris 06, F-29680 Roscoff, France.
C2 IRSA CNR, ITALY
   INST MAX PLANCK, GERMANY
   CNRS, FRANCE
   UNIV PARIS 06, FRANCE
IF 2.393
TC 27
UR https://archimer.ifremer.fr/doc/00488/59948/63196.pdf
   https://archimer.ifremer.fr/doc/00488/59948/63197.pdf
LA English
DT Article
DE ;Coastal lake ecology;Planctomycetes;Bacterial diversity;FISH;CARD-FISH;16S rRNA gene sequencing
AB The phylum Planctomycetes is involved in important processes, such as the mineralization of algal biomass and the removal of nitrogen. Using a combination of 16S rRNA sequence analysis and in situ hybridization, we analyzed the diversity and dynamics of Planctomycetes in a shallow meso-eutrophic lake, Lago di Paola, Italy. Planctomycetes detected by the probe PLA46 accounted for 1 to 5% of prokaryotic picoplankton. Abundances were higher in the coastal lake than in the adjacent marine waters. In the surface waters of the lake, the numbers of Planctomycetes fluctuated greatly, reaching a maximum of 1.5 x 10(6) cells ml(-1) in July. The hypoxic bottom waters had less variable cell abundances. The Planctomycetes counts were positively correlated with chlorophyll a concentrations, confirming the role of this phylum in the degradation of algal biomass. We obtained 70 almost full-length 16S rRNA gene sequences of Planctomycetes from 2 libraries. Four distinct clades could be identified. The Pirellula-related group F and the uncultured Planctomycetes group B both had the highest identity with sequences retrieved from marine habitats, whereas the Pirellula-related group E was affiliated with sequences known from freshwater and brackish water environments. The Planctomyces-related group A seems to have a wide habitat range. Catalyzed reported deposition fluorescence in situ hybridization with newly developed probes revealed abundances of the 4 clades in surface and bottom waters ranging from 1 x 10(3) to 5 x 10(4) cells ml(-1). Together, the 4 clade-specific probes identified only about a quarter of the Planctomycetes detected by probe PLA46. This indicates that the diversity of Planctomycetes has not yet been fully explored.
PY 2011
SO Aquatic Microbial Ecology
SN 0948-3055
PU Inter-research
VL 65
IS 2
UT 000297975700003
DI 10.3354/ame01535
ID 59948
ER

EF