FN Archimer Export Format
PT J
TI Carbon Orientation in the Diatom Phaeodactylum tricornutum: The Effects of Carbon Limitation and Photon Flux Density
BT 
AF Heydarizadeh, Parisa
   Veidi, Brigitte
   Huang, Bing
   LUKOMSKA, Ewa
   Wielgosz-Collin, Gaetane
   Couzinet-Mossion, Aurélie
   BOUGARAN, Gael
   Marchand, Justine
   Schoefs, Benoit
AS 1:1;2:1;3:1;4:2;5:3;6:3;7:2;8:1;9:1;
FF 1:;2:;3:;4:PDG-RBE-BRM-LPBA;5:;6:;7:PDG-RBE-BRM-LPBA;8:;9:;
C1 Metabolism, Bioengineering of Microalgal Molecules and Applications, Mer Molécule Santé, Le Mans University, IUML FR 3473 CNRS, Le Mans, France
   Physiology and Biotechnology of Algae Laboratory, IFREMER, Nantes, France
   Mer Molécule Santé, University of Nantes, IUML FR 3473 CNRS, Nantes, France
C2 UNIV LE MANS, FRANCE
   IFREMER, FRANCE
   UNIV NANTES, FRANCE
SI NANTES
SE PDG-RBE-BRM-LPBA
IN WOS Ifremer UPR
   DOAJ
   copubli-france
   copubli-univ-france
IF 4.402
TC 22
UR https://archimer.ifremer.fr/doc/00489/60058/63338.pdf
   https://archimer.ifremer.fr/doc/00489/60058/63339.pdf
   https://archimer.ifremer.fr/doc/00489/60058/63340.pdf
   https://archimer.ifremer.fr/doc/00489/60058/63341.pdf
LA English
DT Article
DE ;diatom;carbon deficiency;carbon metabolism;stress;light intensity;regulation;biotechnology;phosphoenolpyruvate
AB Diatoms adapt to changing environmental conditions in very efficient ways. Among the mechanisms that can be activated, the reorientation of carbon metabolism is crucial because it allows the storage of energy into energy-dense molecules, typically lipids. Beside their roles in physiology, lipids are commercially interesting compounds. Therefore studies dealing with this topic are relevant for both basic and applied science. Although the molecular mechanisms involved in the reorientation of carbon metabolism as a response to a deficiency in nutrients such as nitrogen or phosphorus has been partially elucidated, the impacts of carbon availability on the implementation of the reorientation mechanisms remain unclear. Indeed, it has not been determined if the same types of mechanisms are activated under carbon and other nutrient deficiencies or limitations. The first aim of this work was to get insights into the physiological, biological and molecular processes triggered by progressive carbon starvation in the model diatom Phaeodactylum tricornutum. The second aim was to investigate the effects of the growth light intensity on these processes. For such a purpose three different photon flux densities 30, 300, and 1000 μmol photons m-2 s-1 were used. The results presented here demonstrate that under carbon limitation, diatom cells still reorient carbon metabolism toward either phosphoenolpyruvate or pyruvate, which serves as a hub for the production of more complex molecules. The distribution of carbon atoms between the different pathways was partially affected by the growth photon flux density because low light (LL) provides conditions for the accumulation of chrysolaminarin, while medium light mostly stimulated lipid synthesis. A significant increase in the amount of proteins was observed under high light (HL). 
PY 2019
PD APR
SO Frontiers In Plant Science
SN 1664-462X
PU Frontiers Media SA
VL 10
IS 471
UT 000464920700001
DI 10.3389/fpls.2019.00471
ID 60058
ER

EF