Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore

Type Article
Date 2018-03
Language English
Author(s) Gobet AngeliqueORCID1, Mest Laetitia1, Perennou Morgan2, Dittami Simon M.1, Caralp Claire3, Coulombet Celine4, Huchette Sylvain4, Roussel Sabine3, Michel Gurvan1, Leblanc Catherine1
Affiliation(s) 1 : Sorbonne Univ, UPMC Univ Paris 06, Integrat Biol Marine Models, CNRS,UMR 8227,Stn Biol Roscoff, CS 90074, F-29688 Roscoff, France.
2 : Sorbonne Univ, UPMC Univ Paris 06, Stn Biol Roscoff, CNRS,FR2424,Genomer, CS 90074, F-29688 Roscoff, France.
3 : IUEM, UMR 6539, LEMAR, Plouzane, France.
4 : France Haliotis, Plouguerneau, France.
Source Microbiome (2049-2618) (Biomed Central Ltd), 2018-03 , Vol. 6 , N. 60 14p. , P. -
DOI 10.1186/s40168-018-0430-7
WOS© Times Cited 8
Keyword(s) Microbe-host interactions, Digestive microbiota, Abalone, Macroalgae, Holobiont
Abstract

Background: Holobionts have a digestive microbiota with catabolic abilities allowing the degradation of complex dietary compounds for the host. In terrestrial herbivores, the digestive microbiota is known to degrade complex polysaccharides from land plants while in marine herbivores, the digestive microbiota is poorly characterized. Most of the latter are generalists and consume red, green, and brown macroalgae, three distinct lineages characterized by a specific composition in complex polysaccharides, which represent half of their biomass. Subsequently, each macroalga features a specific epiphytic microbiota, and the digestive microbiota of marine herbivores is expected to vary with a monospecific algal diet. We investigated the effect of four monospecific diets (Palmaria palmata, Ulva lactuca, Saccharina latissima, Laminaria digitata) on the composition and specificity of the digestive microbiota of a generalist marine herbivore, the abalone, farmed in a temperate coastal area over a year. The microbiota from the abalone digestive gland was sampled every 2 months and explored using metabarcoding.& para;& para;Results: Diversity and multivariate analyses showed that patterns of the microbiota were significantly linked to seasonal variations of contextual parameters but not directly to a specific algal diet Three core genera: Psychrilyobacter, Mycoplasma, and Vibrio constantly dominated the microbiota in the abalone digestive gland. Additionally, a less abundant and diet-specific core microbiota featured genera representing aerobic primary degraders of algal polysaccharides.& para;& para;Conclusions: This study highlights the establishment of a persistent core microbiota in the digestive gland of the abalone since its juvenile state and the presence of a less abundant and diet-specific core community. While composed of different microbial taxa compared to terrestrial herbivores, the digestive gland constitutes a particular niche in the abalone holobiont, where bacteria (i) may cooperate to degrade algal polysaccharides to products assimilable by the host or (ii) may have acquired these functions through gene transfer from the aerobic algal microbiota.

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Publisher's official version 14 2 MB Open access
Additional file 1: Figure S1. Alpha-diversity of the digestive microbiota of abalone, as described by the Shannon (left panels) and Simpson (right ... 9 975 KB Open access
Additional file 2: Table S1. Wilcoxon test to compare Shannon or Simpson indices between algal diets for the whole data set or between sampling dates ... 15 KB Open access
Additional file 3: Table S2. R value of the ANOSIM calculated for the whole dataset and for each algal diet dataset separately between sample groups ... 15 KB Open access
Additional file 4: Table S3. Number of annotated genes for each glycoside hydrolase, polysaccharide lyase, and sulfatase family in all Vibrio, Mycoplasma, Polaribacter, Roseobacter, and ... 160 KB Open access
Additional file 5: Table S4. Genes belonging to families of glycoside hydrolases, polysaccharide lyases, or sulfatases and genes putatively involved in the pyruvate fermentation to acetate pathway... 66 KB Open access
Additional file 6: Table S5. Measures of abalone characteristics and algal composition from April 2012 to January 2013. See supplementary text for ... 33 KB Open access
Additional file 7: Table S6. Fasta sequences of the pyruvate to acetate formation pathways, I, II, and IV, commonly found in Bacteria according to ... 30 221 KB Open access
Additional file 8: Supplementary text. Supplementary information on methods used and results analysed for the study.... 9 200 KB Open access
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How to cite 

Gobet Angelique, Mest Laetitia, Perennou Morgan, Dittami Simon M., Caralp Claire, Coulombet Celine, Huchette Sylvain, Roussel Sabine, Michel Gurvan, Leblanc Catherine (2018). Seasonal and algal diet-driven patterns of the digestive microbiota of the European abalone Haliotis tuberculata, a generalist marine herbivore. Microbiome, 6(60 14p.), -. Publisher's official version : https://doi.org/10.1186/s40168-018-0430-7 , Open Access version : https://archimer.ifremer.fr/doc/00489/60071/