FN Archimer Export Format PT J TI Nitrogen and phosphorus limitations induce carbon partitioning and membrane lipid remodelling in the marine diatom Phaeodactylum tricornutum BT AF Huang, Bing Marchand, Justine Blanckaert, Vincent Lukomska, Ewa Ulmann, Lionel Wielgosz-Collin, Gaëtane Rabesaotra, Vony Moreau, Brigitte Bougaran, Gael Mimouni, Virginie Morant-Manceau, Annick AS 1:1;2:1;3:2;4:3;5:2;6:4;7:4;8:1;9:3;10:2;11:1; FF 1:;2:;3:;4:PDG-RBE-BRM-LPBA;5:;6:;7:;8:;9:PDG-RBE-BRM-LPBA;10:;11:; C1 Laboratoire Mer, Molécules, Santé (IUML – FR 3473 CNRS), UFR Sciences et Techniques, Le Mans Université, avenue Olivier Messiaen, 72085 Le Mans cedex 09, France Laboratoire Mer, Molécules, Santé (IUML – FR 3473 CNRS), IUT Génie Biologique, Le Mans Université, 52 rue des Docteurs Calmette et Guérin, 53020 Laval cedex 9, France Laboratoire Physiologie et Biotechnologie des Algues, IFREMER, rue de l’lle d’Yeu, BP 21105, 44311 Nantes Cedex 03, France Laboratoire Mer, Molécules, Santé (IUML – FR 3473 CNRS), UFR Sciences pharmaceutiques et biologiques, Université de Nantes, 9 rue Bias, 44035 Nantes, France C2 UNIV LE MANS, FRANCE UNIV LE MANS, FRANCE IFREMER, FRANCE UNIV NANTES, FRANCE SI NANTES SE PDG-RBE-BRM-LPBA IN WOS Ifremer UPR copubli-france copubli-univ-france IF 2.756 TC 30 UR https://archimer.ifremer.fr/doc/00491/60249/63861.pdf LA English DT Article DE ;Betaine lipid;lipid remodelling;microalga;nitrogen;phosphorus;polyunsaturated fatty acid AB Nitrogen (N) and phosphorus (P) limitations induce triacylglycerol (TAG) accumulation and membrane lipid remodelling in the marine diatom Phaeodactylum tricornutum. However, a clear understanding of the metabolic reorientation is still lacking. Carbon partitioning is of great interest because this microalga produces various highly valuable molecules such as lipids and polyunsaturated fatty acids. This study compared growth, photosynthetic activity, biochemical and transcriptional responses of P. tricornutum throughout batch culture under N or P limitation. The integrated results show that the photosynthetic intensity was greatly reduced under N or P limitation. Under N limitation, the degradation and re-use of cellular N-containing compounds contributed to TAG accumulation, whilst P limitation favoured TAG accumulation due to the efficiency of carbon fixation, without massive degradation of essential compounds at cellular level. There was no difference in the partitioning of carbon to neutral lipids between N and P limitation. Substitution of phospholipids with betaine lipids appeared to be a P-specific acclimation strategy in P. tricornutum, which was largely regulated at the gene expression level. Betaine lipid synthesis was induced by P limitation. The lipid remodelling began once the medium became deficient in P. While the phospholipid biosynthesis pathway was not completely inhibited, a shift of lipid classes occurred immediately after their synthesis via phospholipid-recycling mechanisms. PY 2019 PD JUN SO European Journal Of Phycology SN 0967-0262 PU Informa UK Limited VL 54 IS 3 UT 000469699000001 BP 342 EP 358 DI 10.1080/09670262.2019.1567823 ID 60249 ER EF