FN Archimer Export Format PT J TI Betaine lipid and neutral lipid production under nitrogen or phosphorus limitation in the marine microalga Tisochrysis lutea (Haptophyta) BT AF Huang, Bing Marchand, Justine Thiriet-Rupert, Stanislas Carrier, Gregory Saint-Jean, Bruno Lukomska, Ewa Moreau, Brigitte Morant-Manceau, Annick Bougaran, Gael Mimouni, Virginie AS 1:1;2:1;3:1;4:2;5:2;6:2;7:1;8:1;9:2;10:3; FF 1:;2:;3:;4:PDG-RBE-BRM-LPBA;5:PDG-RBE-BRM-LPBA;6:PDG-RBE-BRM-LPBA;7:;8:;9:PDG-RBE-BRM-LPBA;10:; C1 Laboratoire Mer, Molécules, Santé (IUML – FR 3473 CNRS), UFR Sciences et Techniques, Le Mans Université, avenue Olivier Messiaen, 72085 Le Mans cedex 09, France Laboratoire Physiologie et Biotechnologie des Algues, IFREMER, rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 03, France Laboratoire Mer, Molécules, Santé (IUML – FR 3473 CNRS), IUT Département Génie Biologique, Le Mans Université, 52 rue des Docteurs Calmette et Guérin, 53020 Laval cedex 9, France C2 UNIV LE MANS, FRANCE IFREMER, FRANCE UNIV LE MANS, FRANCE SI NANTES SE PDG-RBE-BRM-LPBA IN WOS Ifremer UPR copubli-france copubli-univ-france IF 4.008 TC 36 UR https://archimer.ifremer.fr/doc/00492/60409/64776.pdf LA English DT Article DE ;Tisochrysis lutea;Phosphorus;Nitrogen;Lipid remodeling;Betaine lipid;Polyunsaturated fatty acid AB Nitrogen (N) and phosphorus (P) limitations induce neutral lipid accumulation and membrane lipid remodeling in the domesticated oleaginous microalga Tisochrysis lutea. This study compared growth, photosynthetic activity, biochemical and transcriptional responses of T. lutea throughout batch cultures under N or P limitation compared with non-limiting nutrient condition (NP). The results show that, under N limitation, the breakdown and re-use of cellular N-containing compounds contributed to carbohydrates and further triacylglycerol (TAG) accumulation, where the accumulation of docosahexaenoic acid (DHA) in neutral lipids might mainly be derived from the recycling of membrane polar lipids. Conversely, P limitation did not hinder the photosynthetic capacity; a higher efficiency of carbon fixation fueled the allocation of carbon fluxes to the reserves of carbohydrates and neutral lipids. These latter accumulated without massive degradation of essential cellular compounds. Betaine lipids constitute the major compounds of non-plastidial membranes in T. lutea. Given an extremely low constitutive phospholipid level, phospholipids might not be involved in the regulation of P storage. However, transfer of P from non-plastidial to chloroplast membranes might occur, keeping a stable lipid composition of thylakoid membranes and maintaining a relatively high photosynthetic capacity under P deprivation. PY 2019 PD JUL SO Algal Research-biomass Biofuels And Bioproducts SN 2211-9264 PU Elsevier BV VL 40 UT 000468144100030 DI 10.1016/j.algal.2019.101506 ID 60409 ER EF