FN Archimer Export Format
PT J
TI Chemosynthetic ectosymbionts associated with a shallow-water marine nematode
BT 
AF Bellec, Laure
   CAMBON BONAVITA, Marie-Anne
   Hourdez, Stéphane
   Jebbar, Mohamed
   Tasiemski, Aurélie
   Durand, Lucile
   Gayet, Nicolas
   Zeppilli, Daniela
AS 1:1,2,3,4;2:2,3,4;3:5;4:3,4;5:6;6:2,3,4;7:1;8:1;
FF 1:;2:PDG-REM-EEP-LMEE;3:;4:;5:;6:PDG-REM-EEP-LMEE;7:PDG-REM-EEP-LEP;8:PDG-REM-EEP-LEP;
C1 IFREMER, Centre Brest, REM/EEP/LEP, ZI de la pointe du diable, CS10070, 29280, Plouzané, France
   IFREMER, Univ Brest, CNRS, Laboratoire de Microbiologie des Environnements Extrêmes, F-29280, Plouzané, France
   CNRS, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Institut Universitaire Européen de la Mer (IUEM), Technopole Brest-Iroise, Plouzané, France
   Université Bretagne Occidentale (UBO), UMR 6197 - Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Institut Universitaire Européen de la Mer (IUEM), Technopole Brest-Iroise, Plouzané, France
   Station biologique de Roscoff, UMR 7144 CNRS-SU, Adaptation and Biology of Invertebrates in Extreme Environment team, Place G. Teissier, 29680, Roscoff, France
   Université Lille, CNRS, UMR 8198 - Evo-Eco-Paleo, SPICI group, 59000, Lille, France
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   CNRS, FRANCE
   UBO, FRANCE
   CNRS, FRANCE
   UNIV LILLE, FRANCE
SI BREST
SE PDG-REM-EEP-LMEE
   PDG-REM-EEP-LEP
UM BEEP-LM2E
IN WOS Ifremer UPR
   WOS Ifremer UMR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-univ-france
IF 3.998
TC 12
UR https://archimer.ifremer.fr/doc/00494/60614/64098.pdf
   https://archimer.ifremer.fr/doc/00494/60614/64099.pdf
LA English
DT Article
AB Prokaryotes and free-living nematodes are both very abundant and co-occur in marine environments, but little is known about their possible association. Our objective was to characterize the microbiome of a neglected but ecologically important group of free-living benthic nematodes of the Oncholaimidae family. We used a multi-approach study based on microscopic observations (Scanning Electron Microscopy and Fluorescence In Situ Hybridization) coupled with an assessment of molecular diversity using metabarcoding based on the 16S rRNA gene. All investigated free-living marine nematode specimens harboured distinct microbial communities (from the surrounding water and sediment and through the seasons) with ectosymbiosis seemed more abundant during summer. Microscopic observations distinguished two main morphotypes of bacteria (rod-shaped and filamentous) on the cuticle of these nematodes, which seemed to be affiliated to Campylobacterota and Gammaproteobacteria, respectively. Both ectosymbionts belonged to clades of bacteria usually associated with invertebrates from deep-sea hydrothermal vents. The presence of the AprA gene involved in sulfur metabolism suggested a potential for chemosynthesis in the nematode microbial community. The discovery of potential symbiotic associations of a shallow-water organism with taxa usually associated with deep-sea hydrothermal vents, is new for Nematoda, opening new avenues for the study of ecology and bacterial relationships with meiofauna. 
PY 2019
PD MAY
SO Scientific Reports
SN 2045-2322
PU Springer Science and Business Media LLC
VL 9
IS 1
UT 000467137300018
DI 10.1038/s41598-019-43517-8
ID 60614
ER

EF