FN Archimer Export Format
PT J
TI Prevalence, Molecular Identification, and Risk Factors for Cryptosporidium Infection in Edible Marine Fish: A Survey Across Sea Areas Surrounding France
BT 
AF Certad, Gabriela
   Follet, Jérôme
   Gantois, Nausicaa
   Hammouma-Ghelboun, Ourida
   Guyot, Karine
   Benamrouz-Vanneste, Sadia
   Fréalle, Emilie
   Seesao, Yuwalee
   Delaire, Baptiste
   Creusy, Colette
   Even, Gaël
   Verrez-Bagnis, Veronique
   Ryan, Una
   Gay, Mélanie
   Aliouat-Denis, Cécile
   Viscogliosi, Eric
AS 1:1,2;2:3,4;3:1;4:3;5:1;6:1,5;7:1;8:1;9:6;10:6;11:7,8;12:9;13:10;14:11;15:1;16:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:PDG-RBE-BRM-LEMMMB;13:;14:;15:;16:;
C1 CNRS, Inserm, CHU Lille, U1019 – UMR 8204 – CIIL – Centre d’Infection et d’Immunité de Lille, Institut Pasteur de Lille, Université de Lille, Lille, France
   Délégation à la Recherche Clinique et à l’innovation, Groupement des Hôpitaux de l’Institut Catholique de Lille, Lille, France
   ISA-YNCREA Hauts-de-France, Lille, France
   CNRS, ISEN, UMR 8520 – IEMN, Université de Lille, Lille, France
   Laboratoire Ecologie et Biodiversité, Faculté de Gestion Economie et Sciences, Institut Catholique de Lille, Lille, France
   Service d’Anatomie et de Cytologie Pathologiques, Groupement des Hôpitaux de l’Institut Catholique de Lille, Lille, France
   Gènes Diffusion, Douai, France
   PEGASE-Biosciences, Institut Pasteur de Lille, Lille, France
   Ifremer, Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies, Nantes, France
   Centre for Sustainable Aquatic Ecosystems, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, Australia
   Laboratory for Food Safety, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Boulogne-sur-mer, France
C2 CNRS, FRANCE
   INST CATHOL LILLE, FRANCE
   ISA-YNCREA, FRANCE
   CNRS, FRANCE
   INST CATHOL LILLE, FRANCE
   INST CATHOL LILLE, FRANCE
   GÈNES DIFFUSION, FRANCE
   INST PASTEUR, FRANCE
   IFREMER, FRANCE
   UNIV MURDOCH, AUSTRALIA
   ANSES, FRANCE
SI NANTES
SE PDG-RBE-BRM-LEMMMB
IN WOS Ifremer UPR
   DOAJ
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 4.235
TC 27
UR https://archimer.ifremer.fr/doc/00497/60864/64198.pdf
   https://archimer.ifremer.fr/doc/00497/60864/64199.tiff
   https://archimer.ifremer.fr/doc/00497/60864/64200.tiff
LA English
DT Article
CR EVHOE EVALUATION HALIEUTIQUE OUEST DE L'EUROPE
   IBTS INTERNATIONAL BOTTOM TRAWL SURVEY (IBTS),
   PELGAS
   PELMED - PELAGIQUES MEDITERRANÉE
DE ;Cryptosporidium;edible marine fish;18S rRNA gene;gp60;molecular epidemiology;phylogeny;novel genotypes;European seas
AB Cryptosporidium, a zoonotic pathogen, is able to infect a wide range of hosts including wild and domestic animals, and humans. Although it is well known that some parasites are both fish pathogens and recognized agents of zoonosis with a public health impact, little information is available concerning the prevalence of Cryptosporidium in wild aquatic environments. To evaluate the prevalence of Cryptosporidium spp. in commercially important edible marine fish in different European seas (English channel, North sea, Bay of Biscay, Celtic sea and Mediterranean sea), 1,853 specimens were collected as part of two surveys. Nested PCR followed by sequence analysis at the 18S rRNA gene locus was used to identify Cryptosporidium spp. The overall prevalence of Cryptosporidium spp. in sampled fish reached 2.3% (35 out of 1,508) in a first campaign and 3.2% (11 out of 345) in a second campaign. Sequence and phylogenetic analysis of positive samples identified Cryptosporidium parvum (n = 10) and seven genotypes which exhibited between 7.3 and 10.1% genetic distance from C. molnari, with the exception of one genotype which exhibited only 0.5–0.7% genetic distance from C. molnari. Among 31 analyzed fish species, 11 (35.5%) were identified as potential hosts for Cryptosporidium. A higher prevalence of Cryptosporidium spp. was observed in larger fish, in fish collected during the spring-summer period, and in those caught in the North East Atlantic. Pollachius virens (saithe) was the most frequently Cryptosporidium positive species. In fish infected by other parasites, the risk of being Cryptosporidium positive increased 10-fold (OR: 9.95, CI: 2.32–40.01.04, P = 0.0002). Four gp60 subtypes were detected among the C. parvum positive samples: IIaA13G1R1, IIaA15G2R1, IIaA17G2R1, and IIaA18G3R1. These C. parvum subtypes have been previously detected in terrestrial mammals and may constitute an additional source of infection for other animals and in particular for humans. Microscopical examination of histological sections confirmed the presence of round bodies suggestive of the development of C. parvum within digestive glands. We report herein the first epidemiological and molecular data concerning the detection of Cryptosporidium in edible marine fish in European seas surrounding France broadening its host range and uncovering potential novel infection routes. 
PY 2019
PD MAY
SO Frontiers In Microbiology
SN 1664-302X
PU Frontiers Media SA
VL 10
IS 1037
UT 000467956800001
DI 10.3389/fmicb.2019.01037
ID 60864
ER

EF