FN Archimer Export Format
PT J
TI Gill transcriptomic analysis in fast- and slow-growing individuals of Mytilus galloprovincialis
BT 
AF Prieto, Daniel
   Markaide, Pablo
   Urrutxurtu, Iñaki
   Navarro, Enrique
   Artigaud, Sebastien
   Fleury, Elodie
   Ibarrola, Irrintzi
   Urrutia, Miren Bego
AS 1:1;2:1;3:1;4:1;5:2;6:3;7:1;8:1;
FF 1:;2:;3:;4:;5:;6:PDG-RBE-PFOM-LPI;7:;8:;
C1 GIU 17/061, GI 544, Departamento de Genética, Antropología Física y Fisiología Animal, Facultad de Ciencia y Tecnología, Universidad del País Vasco/Euskal Herriko Unibertsitatea, UPV/EHU, Apartado 644, 48080 Bilbao, Spain
   LEMAR UMR 6539 CNRS/UBO/IRD/Ifremer, IUEM, rue Dumont d'Urville, 29280 Plouzané, France
   LEMAR, UMR 6539 UBO-CNRS-Ifremer-IRD, Technopole Brest Iroise 29280 Plouzané, France
C2 UNIV PAIS VASCO EHU, SPAIN
   UBO, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-RBE-PFOM-LPI
UM LEMAR
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   copubli-france
   copubli-europe
   copubli-univ-france
IF 3.224
TC 13
UR https://archimer.ifremer.fr/doc/00503/61490/65293.pdf
LA English
DT Article
DE ;Fast-growing;Mussel;Gill;Transcriptome
AB The molecular basis underlying the mechanisms at the origin of growth variation in bivalves is still poorly understood, although several genes have been described as upregulated in fast-growing individuals. In the present study, we reared mussel spat of the species Mytilus galloprovincialis under diets below the pseudofaeces threshold (BP) and above the pseudofaeces threshold (AP). After 3 months, F and S mussels from each condition were selected to obtain 4 experimental groups: FBP, SBP, FAP and SAP. We hypothesized that the nurturing conditions during the growing period would modify the molecular basis of their growth rate differences. To decipher the molecular mechanisms underlying the growth variation, the gill transcriptomes for the four mussel groups were analysed. Gene expression analysis revealed i) a low number (12) of genes differentially expressed in association with diet and ii) 117 genes differentially expressed by the fast- and slow-growing mussels. According to Biological Process GO term analysis transcriptomic differences between the F and S mussels were mainly based on the upregulation of: response to the stimulus, growth and cell activity. Regarding the KEGG terms, carbohydrate metabolism and the Krebs cycle were upregulated in F mussels, whereas biosynthetic processes were upregulated in S mussels. In accordance with their larger gill surface area and higher rates of feeding and growth, the F individuals overexpressed genes in their gill tissues, and these were involved in i) growth (insulin-like growth factors and myostatin); ii) maintenance of the structure and functioning of extracellular matrix (collagen, laminin, fibulin and decorin); iii) filtration and ciliary activity (mucin, fibrocystin, dynein and tilB homolog protein genes); iv) aerobic metabolism (citrate synthase and carbonic anhydrase); and v) the immune-system, probably in association with haemocytes. In contrast, S individuals overexpressed a different series of genes pertaining to immune system (leucine-rich repeat protein and galectin), along with genes involved in the response to cellular stress (Heat shock protein (HSP24) and metalloendopeptidase) as well as anaerobic metabolism (C4-dicarboxylate transporter). These results might suggest that S individuals would have a greater prevalence of pathogens/diseases or a higher susceptibility to the pathogens. 
PY 2019
PD SEP
SO Aquaculture
SN 0044-8486
PU Elsevier BV
VL 511
UT 000480233600050
DI 10.1016/j.aquaculture.2019.734242
ID 61490
ER

EF