Direct evidence for toxin production by Pseudo-nitzschia plurisecta (Bacillariophyceae) and extension of its distribution area

Type Article
Date 2019-10
Language English
Author(s) Caruana AmandineORCID1, Ayache Nour1, Raimbault Virginie1, Rétho Michael2, Hervé FabienneORCID1, Bilien GwenaelORCID3, Amzil Zouher1, Chomérat Nicolas3
Affiliation(s) 1 : IFREMER – Phycotoxin Laboratory, BP 21105, F – 44311 Nantes, France
2 : IFREMER/LER-MPL, BP 86, F – 56470 La Trinité sur Mer, France
3 : IFREMER/LER-BO, BP 40537, F – 29185 Concarneau, France
Source European Journal Of Phycology (0967-0262) (Informa UK Limited), 2019-10 , Vol. 54 , N. 4 , P. 585-594
DOI 10.1080/09670262.2019.1620342
Keyword(s) Coastal monitoring, domoic acid, pigment composition, Pseudo-nitzschia plurisecta, taxonomic identification, toxic diatoms
Abstract

We confirm an amnesic shellfish poisoning risk associated with domoic acid production by the species Pseudo-nitzschia plurisecta and determine the toxin profile, which includes three domoic acid isomers. Although the species was reported once in European waters, along the Spanish Atlantic coast, here we describe and characterize P. plurisecta collected during the first occurrence in French Atlantic waters. Three strains (IFR-PPL-15.1, 15.2 and 15.3) were isolated from French coastal water samples in June 2015. These strains were identified and characterized by microscopic observation, molecular (ITS region sequencing and phylogenetic analysis) and metabolite (toxins and pigments) analyses. The cell shape is lanceolate and the valve contains a central interspace with uniseriate stria of oval-square poroids, each divided into 3–10 sectors. The ITS gene sequence is homologous to the original sequence available for the Spanish strain P. plurisecta. The pigment profile contains chl a, c3, c2, fucoxanthin, diatoxanthin, diadinoxanthin and traces of β,β-carotene. Results on morphology, phylogeny and pigments led to the identification of P. plurisecta and confirmed its first occurrence in French waters.

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Caruana Amandine, Ayache Nour, Raimbault Virginie, Rétho Michael, Hervé Fabienne, Bilien Gwenael, Amzil Zouher, Chomérat Nicolas (2019). Direct evidence for toxin production by Pseudo-nitzschia plurisecta (Bacillariophyceae) and extension of its distribution area. European Journal Of Phycology, 54(4), 585-594. Publisher's official version : https://doi.org/10.1080/09670262.2019.1620342 , Open Access version : https://archimer.ifremer.fr/doc/00505/61687/