TY - JOUR T1 - Direct evidence for toxin production by Pseudo-nitzschia plurisecta (Bacillariophyceae) and extension of its distribution area A1 - Caruana,Amandine A1 - Ayache,Nour A1 - Raimbault,Virginie A1 - Rétho,Michael A1 - Hervé,Fabienne A1 - Bilien,Gwenael A1 - Amzil,Zouher A1 - Chomérat,Nicolas AD - IFREMER – Phycotoxin Laboratory, BP 21105, F – 44311 Nantes, France AD - IFREMER/LER-MPL, BP 86, F – 56470 La Trinité sur Mer, France AD - IFREMER/LER-BO, BP 40537, F – 29185 Concarneau, France UR - https://doi.org/10.1080/09670262.2019.1620342 DO - 10.1080/09670262.2019.1620342 KW - Coastal monitoring KW - domoic acid KW - pigment composition KW - Pseudo-nitzschia plurisecta KW - taxonomic identification KW - toxic diatoms N2 - We confirm an amnesic shellfish poisoning risk associated with domoic acid production by the species Pseudo-nitzschia plurisecta and determine the toxin profile, which includes three domoic acid isomers. Although the species was reported once in European waters, along the Spanish Atlantic coast, here we describe and characterize P. plurisecta collected during the first occurrence in French Atlantic waters. Three strains (IFR-PPL-15.1, 15.2 and 15.3) were isolated from French coastal water samples in June 2015. These strains were identified and characterized by microscopic observation, molecular (ITS region sequencing and phylogenetic analysis) and metabolite (toxins and pigments) analyses. The cell shape is lanceolate and the valve contains a central interspace with uniseriate stria of oval-square poroids, each divided into 3–10 sectors. The ITS gene sequence is homologous to the original sequence available for the Spanish strain P. plurisecta. The pigment profile contains chl a, c3, c2, fucoxanthin, diatoxanthin, diadinoxanthin and traces of β,β-carotene. Results on morphology, phylogeny and pigments led to the identification of P. plurisecta and confirmed its first occurrence in French waters. Y1 - 2019/10 PB - Informa UK Limited JF - European Journal Of Phycology SN - 0967-0262 VL - 54 IS - 4 SP - 585 EP - 594 ID - 61687 ER -