FN Archimer Export Format PT J TI Direct evidence for toxin production by Pseudo-nitzschia plurisecta (Bacillariophyceae) and extension of its distribution area BT AF Caruana, Amandine Ayache, Nour Raimbault, Virginie Rétho, Michael Hervé, Fabienne Bilien, Gwenael Amzil, Zouher Chomérat, Nicolas AS 1:1;2:1;3:1;4:2;5:1;6:3;7:1;8:3; FF 1:PDG-ODE-DYNECO-PHYC;2:PDG-ODE-DYNECO-PHYC;3:PDG-ODE-DYNECO-PHYC;4:PDG-ODE-LITTORAL-LERMPL;5:PDG-ODE-DYNECO-PHYC;6:PDG-ODE-LITTORAL-LERBO;7:PDG-ODE-DYNECO-PHYC;8:PDG-ODE-LITTORAL-LERBO; C1 IFREMER – Phycotoxin Laboratory, BP 21105, F – 44311 Nantes, France IFREMER/LER-MPL, BP 86, F – 56470 La Trinité sur Mer, France IFREMER/LER-BO, BP 40537, F – 29185 Concarneau, France C2 IFREMER, FRANCE IFREMER, FRANCE IFREMER, FRANCE SI NANTES LORIENT CONCARNEAU SE PDG-ODE-DYNECO-PHYC PDG-ODE-LITTORAL-LERMPL PDG-ODE-LITTORAL-LERBO IN WOS Ifremer UPR IF 2.756 TC 6 UR https://archimer.ifremer.fr/doc/00505/61687/66033.pdf LA English DT Article DE ;Coastal monitoring;domoic acid;pigment composition;Pseudo-nitzschia plurisecta;taxonomic identification;toxic diatoms AB We confirm an amnesic shellfish poisoning risk associated with domoic acid production by the species Pseudo-nitzschia plurisecta and determine the toxin profile, which includes three domoic acid isomers. Although the species was reported once in European waters, along the Spanish Atlantic coast, here we describe and characterize P. plurisecta collected during the first occurrence in French Atlantic waters. Three strains (IFR-PPL-15.1, 15.2 and 15.3) were isolated from French coastal water samples in June 2015. These strains were identified and characterized by microscopic observation, molecular (ITS region sequencing and phylogenetic analysis) and metabolite (toxins and pigments) analyses. The cell shape is lanceolate and the valve contains a central interspace with uniseriate stria of oval-square poroids, each divided into 3–10 sectors. The ITS gene sequence is homologous to the original sequence available for the Spanish strain P. plurisecta. The pigment profile contains chl a, c3, c2, fucoxanthin, diatoxanthin, diadinoxanthin and traces of β,β-carotene. Results on morphology, phylogeny and pigments led to the identification of P. plurisecta and confirmed its first occurrence in French waters. PY 2019 PD OCT SO European Journal Of Phycology SN 0967-0262 PU Informa UK Limited VL 54 IS 4 UT 000474657500001 BP 585 EP 594 DI 10.1080/09670262.2019.1620342 ID 61687 ER EF