FN Archimer Export Format
PT J
TI Evolution of Gene Expression during a Transition from Environmental to Genetic Sex Determination
BT 
AF MOLINIER, Cecile
   REISSER, Celine
   FIELDS, Peter D.
   SEGARD, Adeline
   GALIMOV, Yan
   HAAG, Christoph R.
AS 1:1;2:1,2,3;3:4;4:1;5:5;6:1,2;
FF 1:;2:PDG-RBE-RMPF;3:;4:;5:;6:;
C1 Univ Montpellier, Univ Paul Valery Montpellier 3, CNRS, EPHE,CEFE,IRD, Montpellier, France.
   Univ Fribourg, Ecol & Evolut, Fribourg, Switzerland.
   IFREMER, Ctr Pacifique, UMR EIO 241, Labex CORAIL, Tahiti, French Polynesi, France.
   Univ Basel, Inst Zool, Evolutionary Biol, Basel, Switzerland.
   RAS, Koltsov Inst Dev Biol, Moscow, Russia.
C2 UNIV MONTPELLIER, FRANCE
   UNIV FRIBOURG, SWITZERLAND
   IFREMER, FRANCE
   UNIV BASEL, SWITZERLAND
   RUSSIAN ACAD SCI, RUSSIA
SI TAHITI
SE PDG-RBE-RMPF
UM EIO
IN WOS Ifremer UMR
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 11.062
TC 4
UR https://archimer.ifremer.fr/doc/00506/61744/65766.pdf
   https://archimer.ifremer.fr/doc/00506/61744/84995.zip
LA English
DT Article
DE ;sex chromosomes;differential gene expression;RNA sequencing;genomics;Daphnia magna
AB Genetic sex determination (GSD) can evolve from environmental sex determination (ESD) via an intermediate state in which both coexist in the same population. Such mixed populations are found in the crustacean Daphnia magna, where non-male producers (NMP, genetically determined females) coexist with male producers (MP), in which male production is environmentally inducible and can also artificially be triggered by exposure to juvenile hormone. This makes Daphnia magna a rare model species for the study of evolutionary transitions from ESD to GSD. Although the chromosomal location of the NMP-determining mutation has been mapped, the actual genes and pathways involved in the evolution of GSD from ESD remain unknown. Here, we present a transcriptomic analysis of MP and NMP females under control (female producing) and under hormone exposure conditions. We found similar to 100 differentially expressed genes between MP and NMP under control conditions. Genes in the NMP-determining chromosome region were especially likely to show such constitutive expression differences. Hormone exposure led to expression changes of an additional similar to 100 (MP) to similar to 600 (NMP) genes, with an almost systematic upregulation of those genes in NMP. These observations suggest that the NMP phenotype is not determined by a simple "loss-of-function" mutation. Rather, homeostasis of female offspring production under hormone exposure appears to be an active state, tightly regulated by complex mechanisms involving many genes. In a broader view, this illustrates that the evolution of GSD, while potentially initiated by a single mutation, likely leads to secondary integration involving many genes and pathways. 
PY 2019
PD JUN
SO Molecular Biology And Evolution
SN 0737-4038
PU Oxford Univ Press
VL 36
IS 7
UT 000473589400015
BP 1551
EP 1564
DI 10.1093/molbev/msz123
ID 61744
ER

EF