FN Archimer Export Format
PT J
TI Three-year study to assess human enteric viruses in shellfish
BT 
AF LE GUYADER, Soizick
   HAUGARREAU, Larissa
   MIOSSEC, Laurence
   DUBOIS, Eric
   POMMEPUY, Monique
AS 1:;2:;3:;4:;5:;
FF 1:PDG-DEL-MP-MIC;2:PDG-DEL-MP-MIC;3:PDG-DRV-RA-LGP;4:PDG-DEL-MIC;5:PDG-DEL-MP-MIC;
C1 IFREMER, Microbiol Lab, F-44311 Nantes 03, France.
C2 IFREMER, FRANCE
SI NANTES
   LA TREMBLADE
   BREST
SE PDG-DEL-MP-MIC
   PDG-DRV-RA-LGP
   PDG-DEL-MIC
IN WOS Ifremer jusqu'en 2018
IF 3.389
TC 220
UR https://archimer.ifremer.fr/doc/00507/61879/65953.pdf
   https://archimer.ifremer.fr/doc/00507/61879/65954.pdf
LA English
DT Article
DE ;Molecular technique;Seasonal variation;Human pathology;Viral contamination;Shellfish;Enteric virus
AB The main pathogenic enteric viruses able to persist in the environment, such as hepatitis A virus (HAV), Norwalk-like virus (NLV), enterovirus (EV), rotavirus (RV), and astrovirus (AV), were detected by reverse transcription-PCR and hybridization in shellfish during a 3-year study. Oyster samples (n = 108), occasionally containing bacteria, were less frequently contaminated, showing positivity for AV (17%), NLV (23%), EV (19%), and RV (27%), whereas mussel samples, collected in areas routinely impacted by human sewage, were more highly contaminated: AV (50%), HAV (13%), NLV (35%), EV (45%), and RV (52%). Sequences obtained from HAV and NLV amplicons shelved a great variety of strains, especially for NLV (strains close to Mexico, Snow Mountain Agent, or Norwalk virus), Viral contamination was mainly observed during winter months, although there were some seasonal differences among the viruses. This first study of virus detection over a fairly long period of time suggests that routine analysis of shellfish by a molecular technique is feasible.
PY 2000
PD AUG
SO Applied and environmental microbiology
SN 0099-2240
PU American society for microbiology
VL 66
IS 8
UT 000088546300017
BP 3241
EP 3248
ID 61879
ER

EF