FN Archimer Export Format
PT J
TI Dynamics of the Pacific Oyster Pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next‐generation sequencing strategy
BT 
AF Lasa, Aide
   Cesare, Andrea
   Tassistro, Giovanni
   Borello, Alessio
   Gualdi, Stefano
   Furones, Dolors
   Carrasco, Noelia
   Cheslett, Deborah
   Brechon, Amanda
   Paillard, Christine
   Bidault, Adeline
   Pernet, Fabrice
   Canesi, Laura
   Edomi, Paolo
   Pallavicini, Alberto
   Pruzzo, Carla
   Vezzulli, Luigi
AS 1:1,2;2:3;3:1;4:1;5:4;6:5;7:5;8:6;9:6;10:7;11:7;12:8;13:1;14:9;15:9;16:1;17:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:PDG-RBE-PFOM-LPI;13:;14:;15:;16:;17:;
C1 Department of Earth, Environmental and Life Sciences (DISTAV)University of Genoa Genoa ,Italy
   Department of Microbiology and ParasitologyUniversidade de Santiago de Compostela Santiago de Compostela ,Spain
   National Research Council –Water Research Institute (CNR‐IRSA), Largo Tonolli 50 28822 Verbania ,Italy
   Department of Plant and Microbial BiologyUniversity of Zürich, Switzerland
   IRTA,Sant Carles e la Ràpita Tarragona, Spain
   Fish Health UnitThe Marine Institute, Rinville Oranmore Galway, Ireland
   Laboratoire des sciences de l'Environnement Marin, Institut Universitaire Européen de la MUniversité de Bretagne Occidentale – UMR6539 CNRS/UBO/IRD/Ifremer Plouzané ,France
   Ifremer, Physiologie Fonctionnelle des Organismes MarinsUMR 6539 LEMAR (CNRS/Ifremer/IRD/UBO) Technopole Iroise CS 10070 29280 PLOUZANE, france
   Dept. of Life SciencesUniversity of Trieste, Italy
C2 UNIV GENOA, ITALY
   UNIV SANTIAGO DE COMPOSTELA, SPAIN
   CNR IRSA, ITALY
   UNIV ZURICH, SWITZERLAND
   IRTA, SPAIN
   MARINE INST, IRELAND
   UBO, FRANCE
   IFREMER, FRANCE
   UNIV TRIESTE, ITALY
SI BREST
SE PDG-RBE-PFOM-LPI
UM LEMAR
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   copubli-france
   copubli-europe
   copubli-univ-france
   copubli-int-hors-europe
IF 4.933
TC 47
UR https://archimer.ifremer.fr/doc/00508/61952/66036.pdf
   https://archimer.ifremer.fr/doc/00508/61952/66037.pdf
   https://archimer.ifremer.fr/doc/00508/61952/66038.pdf
   https://archimer.ifremer.fr/doc/00508/61952/66039.pdf
   https://archimer.ifremer.fr/doc/00508/61952/66040.pdf
   https://archimer.ifremer.fr/doc/00508/61952/66041.pdf
   https://archimer.ifremer.fr/doc/00508/61952/66042.txt
LA English
DT Article
AB Infectious agents such as the bacteria Vibrio aestuarianus or Ostreid herpesvirus 1 (OsHV‐1) have been repeatedly associated with dramatic disease outbreaks of Crassostrea gigas beds in Europe. Beside roles played by these pathogens microbial infections in C. gigas may derive from the contribution of a larger number of microorganisms than previously thought, according to an emerging view supporting the polymicrobial nature of bivalve disease. In this study, the microbial communities associated with a large number of C. gigas samples collected during recurrent mortality episodes at different European sites were investigated by real‐time PCR and 16SrRNA gene‐based microbial profiling. A new target enrichment next‐generation sequencing protocol for selective capturing of 884 phylogenetic and virulence markers of the potential microbial pathogenic community in oyster tissue was developed allowing high taxonomic resolution analysis of the bivalve pathobiota. Comparative analysis of contrasting C. gigas samples conducted using these methods revealed that oyster experiencing mortality outbreaks displayed signs of microbiota disruption associated with the presence of previously undetected potential pathogenic microbial species mostly belonging to genus Vibrio and Arcobacter. The role of these species and their consortia should be targeted by future studies aiming to shed light on mechanisms underlying polymicrobial infections in C.gigas. 
PY 2019
PD DEC
SO Environmental Microbiology
SN 1462-2912
PU Wiley
VL 21
IS 12
UT 000481006000001
BP 4548
EP 4562
DI 10.1111/1462-2920.14750
ID 61952
ER

EF