FN Archimer Export Format
PT J
TI Resistance of the oyster pathogen   Vibrio tasmaniensis   LGP32 against grazing by   Vannella sp   . marine amoeba involves Vsm and CopA virulence factors
BT 
AF Robino, Etienne
   Poirier, Aurore C.
   Amraoui, Hajar
   Le Bissonnais, Sandra
   Perret, Angelique
   Lopez‐Joven, Carmen
   Auguet, Jean-Christophe
   Rubio, Tristan P.
   Cazevieille, Chantal
   Rolland, Jean-Luc
   Héchard, Yann
   Destoumieux‐Garzón, Delphine
   Charrière, Guillaume
AS 1:1;2:5;3:5;4:5;5:1;6:;7:2;8:5;9:3;10:1;11:4;12:5;13:5;
FF 1:;2:;3:;4:;5:PDG-RBE-IHPE;6:;7:;8:;9:;10:PDG-RBE-IHPE;11:;12:;13:;
C1 IHPE UMR 5244, CNRS, Ifremer, University of Montpellier, University of Perpignan Via Domitia Montpellier, France
   MARBEC, University of Montpellier, CNRS, Ifremer, IRD Montpellier, France
   INM, University of Montpellier Montpellier, France
   EBI UMR CNRS 7267, University of Poitiers Poitiers, France
   IHPE UMR 5244, CNRS, Ifremer, University of Montpellier, University of Perpignan Via Domitia Montpellier, France
C2 IFREMER, FRANCE
   CNRS, FRANCE
   INM, FRANCE
   UNIV POITIERS, FRANCE
   UNIV MONTPELLIER, FRANCE
SI MONTPELLIER
SE PDG-RBE-IHPE
UM MARBEC
   IHPE
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   copubli-france
   copubli-univ-france
IF 5.491
TC 9
UR https://archimer.ifremer.fr/doc/00509/62109/66292.pdf
LA English
DT Article
AB ibrios are ubiquitous in marine environments and opportunistically colonize a broad range of hosts. Strains of Vibrio tasmaniensis present in oyster farms can thrive in oysters during juvenile mortality events and behave as facultative intracellular pathogen of oyster hemocytes. Herein, we wondered whether V. tasmaniensis LGP32 resistance to phagocytosis is specific to oyster immune cells or contributes to resistance to other phagocytes, like marine amoebae. To address this question, we developed an integrative study, from the first description of amoeba diversity in oyster‐farms to the characterization of LGP32 interactions with amoebae. An isolate of the Vannella genus, Vannella sp. AP1411, which was collected from oyster farms, is ubiquitous, and belongs to one clade of Vannella that could be found associated with Vibrionaceae. LGP32 was shown to be resistant to grazing by Vannella sp. AP1411 and this phenotype depends on some previously identified virulence factors: secreted metalloprotease Vsm and copper efflux p‐ATPase CopA, which act at different steps during amoeba‐vibrio interactions, whereas some other virulence factors were not involved. Altogether, our work indicate that some virulence factors can by involved in multi‐host interactions of V. tasmaniensis ranging from protozoans to metazoans, potentially favoring their opportunistic behavior. 
PY 2020
PD OCT
SO Environmental Microbiology
SN 1462-2912
PU Wiley
VL 22
IS 10
UT 000482838700001
BP 4183
EP 4197
DI 10.1111/1462-2920.14770
ID 62109
ER

EF