FN Archimer Export Format
PT J
TI Selection of  Vibrio crassostreae  relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells
BT 
AF Piel, Damien
   Bruto, Maxime
   James, Adele
   Labreuche, Yannick
   Lambert, Christophe
   Janicot, Adrian
   Chenivesse, Sabine
   Petton, Bruno
   Wegner, K. Mathias
   Stoudmann, Candice
   Blokesch, Melanie
   LE ROUX, Frederique
AS 1:1,2;2:2;3:1,2;4:1,2;5:6;6:2;7:2;8:1,3;9:4;10:5;11:5;12:1,2;
FF 1:PDG-RBE-PFOM;2:PDG-RBE-PFOM;3:PDG-RBE-PFOM;4:PDG-RBE-PFOM;5:;6:;7:;8:PDG-RBE-PFOM-LPI;9:;10:;11:;12:PDG-RBE-PFOM;
C1 Ifremer, Unité Physiologie Fonctionnelle des Organismes Marins, ZI de la Pointe du Diable, C 10070 F‐29280 Plouzané, France
   Sorbonne Universités, UPMC Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074 F‐29688 Roscoff cedex ,France
   Laboratoire des Sciences de l'Environnement Marin (LEMAR), UMR 6539 CNRS UBO IRD IFREMER – Institut Universitaire Européen de la Mer, Technopôle Brest‐Iroise – Rue Dumont d'Urville F‐29280 Plouzané ,France
   AWI ‐ Alfred Wegener Institut ‐ Helmholtz‐Zentrum für Polar‐ und Meeresforschung, Coastal Ecology, Waddensea Station Sylt, Hafenstrasse 43 25992 List, Germany
   Laboratory of Molecular MicrobiologyGlobal Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL) CH‐1015 Lausanne, Switzerland
   Laboratoire des Sciences de l'Environnement Marin (LEMAR), UMR 6539 CNRS UBO IRD IFREMER – Institut Universitaire Européen de la Mer, Technopôle Brest‐Iroise – Rue Dumont d'Urville F‐29280 Plouzané ,France
C2 IFREMER, FRANCE
   UNIV PARIS 06, FRANCE
   IFREMER, FRANCE
   INST A WEGENER, GERMANY
   ECOLE POLYTECH FED LAUSANNE, SWITZERLAND
   UBO, FRANCE
SI ROSCOFF
   BREST
   ARGENTON
SE PDG-RBE-PFOM
   PDG-RBE-PFOM-LPI
UM LEMAR
IN WOS Ifremer UPR
   WOS Ifremer UMR
   WOS Cotutelle UMR
   copubli-france
   copubli-europe
   copubli-univ-france
   copubli-int-hors-europe
IF 5.491
TC 17
UR https://archimer.ifremer.fr/doc/00510/62129/66343.pdf
LA English
DT Article
AB Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae has been repeatedly isolated from diseased animals and the majority of the strains have been demonstrated to be virulent for oysters. In this study we showed that oyster farms exhibited a high prevalence of a virulence plasmid carried by V. crassostreae while oysters, at an adult stage, were reservoirs of this virulent population. The pathogenicity of V. crassostreae depends on a novel transcriptional regulator, which activates the bidirectional promoter of a Type 6 Secretion System (T6SS) genes cluster. Both the T6SS and a second chromosomal virulence factor, r5.7, are necessary for virulence but act independently to cause to hemocyte (oyster immune cell) cytotoxicity. A phylogenetically closely related T6SS was identified in V. aestuarianus and V. tapetis, which infect adult oysters and clams, respectively. We propose that hemocyte cytotoxicity, is a lethality trait shared by a broad range of mollusk pathogens and we speculate that T6SS was involved in parallel evolution of pathogen for mollusks. 
PY 2020
PD OCT
SO Environmental Microbiology
SN 1462-2912
PU Wiley
VL 22
IS 10
UT 000483657800001
BP 4198
EP 4211
DI 10.1111/1462-2920.14776
ID 62129
ER

EF