Transcriptomic analysis of clam extra pallial fluids reveals immunity and cytoskeleton alterations in the first week of Brown Ring Disease development

Type Article
Date 2019-10
Language English
Author(s) Rahmani Alexandra1, Corre Erwan2, Richard Gaëlle1, Bidault Adeline1, Lambert Christophe4, Oliveira Louisi3, Thompson Cristiane3, Thompson Fabiano3, Pichereau Vianney1, Paillard Christine4
Affiliation(s) 1 : Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280, Plouzane, France
2 : Sorbonne Universités, Université Pierre et Marie Curie-Paris 6, CNRS, FR2424, Station Biologique de Roscoff, Roscoff, France
3 : Centro de Ciências da Saúde, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
4 : Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280, Plouzane, France
Source Fish & Shellfish Immunology (1050-4648) (Elsevier BV), 2019-10 , Vol. 93 , P. 940-948
DOI 10.1016/j.fsi.2019.08.025
WOS© Times Cited 3
Keyword(s) Brown ring disease, V. tapetis, R. philippinarum, Hemocytes, Actin cytoskeleton, beta-Thymosin, Coactosin, Resting cells
Abstract

The Brown Ring Disease is an infection caused by the bacterium Vibrio tapetis on the Manila clam Ruditapes philippinarum. The process of infection, in the extrapallial fluids (EPFs) of clams, involves alteration of immune functions, in particular on hemocytes which are the cells responsible of phagocytosis. Disorganization of the actin-cytoskeleton in infected clams is a part of what leads to this alteration. This study is the first transcriptomic approach based on collection of extrapallial fluids on living animals experimentally infected by V. tapetis. We performed differential gene expression analysis of EPFs in two experimental treatments (healthy-against infected-clams by V. tapetis), and showed the deregulation of 135 genes. In infected clams, a downregulation of transcripts implied in immune functions (lysosomal activity and complement- and lectin-dependent PRR pathways) was observed during infection. We also showed a deregulation of transcripts encoding proteins involved in the actin cytoskeleton organization such as an overexpression of β12-Thymosin (which is an actin sequestration protein) or a downregulation of proteins that closely interact with capping proteins such as Coactosin, that counteract action of capping proteins, or Profilin. We validated these transcriptomic results by cellular physiological analyses that showed a decrease of the lysosome amounts and the disorganization of actin cytoskeleton in infected hemocytes.

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Rahmani Alexandra, Corre Erwan, Richard Gaëlle, Bidault Adeline, Lambert Christophe, Oliveira Louisi, Thompson Cristiane, Thompson Fabiano, Pichereau Vianney, Paillard Christine (2019). Transcriptomic analysis of clam extra pallial fluids reveals immunity and cytoskeleton alterations in the first week of Brown Ring Disease development. Fish & Shellfish Immunology, 93, 940-948. Publisher's official version : https://doi.org/10.1016/j.fsi.2019.08.025 , Open Access version : https://archimer.ifremer.fr/doc/00510/62131/