FN Archimer Export Format
PT J
TI Transcriptomic analysis of clam extra pallial fluids reveals immunity and cytoskeleton alterations in the first week of Brown Ring Disease development
BT 
AF Rahmani, Alexandra
   Corre, Erwan
   Richard, Gaëlle
   Bidault, Adeline
   Lambert, Christophe
   Oliveira, Louisi
   Thompson, Cristiane
   Thompson, Fabiano
   Pichereau, Vianney
   Paillard, Christine
AS 1:1;2:2;3:1;4:1;5:4;6:3;7:3;8:3;9:1;10:4;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;
C1 Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280, Plouzane, France
   Sorbonne Universités, Université Pierre et Marie Curie-Paris 6, CNRS, FR2424, Station Biologique de Roscoff, Roscoff, France
   Centro de Ciências da Saúde, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
   Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280, Plouzane, France
C2 UBO, FRANCE
   UNIV PARIS 06, FRANCE
   UNIV FED RIO DE JANEIRO UFRJ, BRAZIL
   CNRS, FRANCE
UM LEMAR
IN WOS Cotutelle UMR
   copubli-france
   copubli-int-hors-europe
   copubli-sud
IF 1.618
TC 5
UR https://archimer.ifremer.fr/doc/00510/62131/66362.pdf
LA English
DT Article
DE ;Brown ring disease;V. tapetis;R. philippinarum;Hemocytes;Actin cytoskeleton;beta-Thymosin;Coactosin;Resting cells
AB The Brown Ring Disease is an infection caused by the bacterium Vibrio tapetis on the Manila clam Ruditapes philippinarum. The process of infection, in the extrapallial fluids (EPFs) of clams, involves alteration of immune functions, in particular on hemocytes which are the cells responsible of phagocytosis. Disorganization of the actin-cytoskeleton in infected clams is a part of what leads to this alteration. This study is the first transcriptomic approach based on collection of extrapallial fluids on living animals experimentally infected by V. tapetis. We performed differential gene expression analysis of EPFs in two experimental treatments (healthy-against infected-clams by V. tapetis), and showed the deregulation of 135 genes. In infected clams, a downregulation of transcripts implied in immune functions (lysosomal activity and complement- and lectin-dependent PRR pathways) was observed during infection. We also showed a deregulation of transcripts encoding proteins involved in the actin cytoskeleton organization such as an overexpression of β12-Thymosin (which is an actin sequestration protein) or a downregulation of proteins that closely interact with capping proteins such as Coactosin, that counteract action of capping proteins, or Profilin. We validated these transcriptomic results by cellular physiological analyses that showed a decrease of the lysosome amounts and the disorganization of actin cytoskeleton in infected hemocytes. 
PY 2019
PD OCT
SO Fish & Shellfish Immunology
SN 1050-4648
PU Elsevier BV
VL 93
UT 000488998300105
BP 940
EP 948
DI 10.1016/j.fsi.2019.08.025
ID 62131
ER

EF