FN Archimer Export Format
PT J
TI Characterization of Norovirus and Other Human Enteric Viruses in Sewage and Stool Samples Through Next-Generation Sequencing
BT 
AF Strubbia, Sofia
   Phan, My V. T.
   Schaeffer, Julien
   Koopmans, Marion
   Cotten, Matthew
   LE GUYADER, Soizick
AS 1:1;2:2;3:1;4:2;5:2;6:1;
FF 1:PDG-RBE-SGMM-LSEM;2:;3:PDG-RBE-SGMM-LSEM;4:;5:;6:PDG-RBE-SGMM-LSEM;
C1 Ifremer, Laboratoire de Microbiologie, LSEM-SG2M, BP 21105, 44311 Nantes Cedex 3, France
   Department of Viroscience, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
C2 IFREMER, FRANCE
   UNIV ROTTERDAM, NETHERLANDS
SI NANTES
SE PDG-RBE-SGMM-LSEM
IN WOS Ifremer UPR
   copubli-europe
IF 2.819
TC 29
UR https://archimer.ifremer.fr/doc/00512/62332/66592.pdf
LA English
DT Article
DE ;Human enteric viruses;Norovirus;Sewage;Metagenomic;Virome
AB This study aimed to optimize a method to identify human enteric viruses in sewage and stool samples using random primed next-generation sequencing. We tested three methods, two employed virus enrichment based on the binding properties of the viral capsid using pig-mucin capture or by selecting viral RNA prior to library preparation through a capture using the SureSelect target enrichment. The third method was based on a non-specific biophysical precipitation with polyethylene glycol. Full genomes of a number of common human enteric viruses including norovirus, rotavirus, husavirus, enterovirus and astrovirus were obtained. In stool samples full norovirus genome were detected as well as partial enterovirus genome. A variety of norovirus sequences was detected in sewage samples, with genogroup II being more prevalent. Interestingly, the pig-mucin capture enhanced not only the recovery of norovirus and rotavirus but also recovery of astrovirus, sapovirus and husavirus. Documenting sewage virome using these methods provides information for molecular epidemiology and may be useful in developing strategies to prevent further spread of viruses. 
PY 2019
PD DEC
SO Food And Environmental Virology
SN 1867-0334
PU Springer Science and Business Media LLC
VL 11
IS 4
UT 000495795000007
BP 400
EP 409
DI 10.1007/s12560-019-09402-3
ID 62332
ER

EF