FN Archimer Export Format
PT J
TI Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)
BT 
AF CORMIER, Bettie
   BATEL, Anika
   CACHOT, Jerome
   BEGOUT, Marie-Laure
   BRAUNBECK, Thomas
   COUSIN, Xavier
   KEITER, Steffen H
AS 1:1,2;2:3;3:2;4:4;5:3;6:5,6;7:1;
FF 1:;2:;3:;4:PDG-RBE-HGS;5:;6:;7:;
C1 Man-Technology-Environment Research Centre, School of Science and Technology, Örebro University, Örebro, Sweden
   University of Bordeaux, EPOC UMR CNRS 5805, Pessac, France
   Aquatic Ecology and Toxicology Group, Center for Organismal Studies, University of Heidelberg, Heidelberg, Germany
   Laboratoire Ressources Halieutiques, IFREMER, L'Houmeau, France
   IFREMER, L3AS, UMR MARBEC, Palavas-les-Flots, France
   UMR GABI INRA, AgroParisTech, University Paris-Saclay, Jouy-en-Josas, France
C2 UNIV OREBRO, SWEDEN
   UNIV BORDEAUX, FRANCE
   UNIV HEIDELBERG, GERMANY
   IFREMER, FRANCE
   IFREMER, L3AS, UMR MARBEC, Palavas-les-Flots, France
   INRA, FRANCE
SI LA ROCHELLE
SE PDG-RBE-HGS
IN WOS Ifremer UPR
   DOAJ
   copubli-france
   copubli-p187
   copubli-europe
   copubli-univ-france
IF 5.411
TC 28
UR https://archimer.ifremer.fr/doc/00514/62545/66896.pdf
   https://archimer.ifremer.fr/doc/00514/62545/66897.pdf
LA English
DT Article
DE ;fish embryotoxicity test (FET);swimming behavior;EROD;cyp1a;perfluorooctane sulfonate;benzo[a]pyrene;oxybenzone
AB As wide-spread pollutants in the marine environment, microplastics (MPs) have raised public concern about potential toxic effects in aquatic organisms, and, among others, MPs were suspected to act as a vector for organic pollutants to biota. The purpose of the present study was to investigate effects by three model pollutants, oxybenzone (BP3), benzo[a]pyrene (BaP), and perfluorooctane sulfonate (PFOS) adsorbed to polyethylene MPs on the basis of a standard assay, the acute fish embryo toxicity test (FET; OECD TG 236) with zebrafish (Danio rerio) supplemented by additional endpoints such as induction of ethoxyresorufin-O-deethylase (EROD) activity, modification of cyp1a gene transcription and changes in larval swimming behavior. FET assays were performed in three laboratories using slightly different husbandry and exposure conditions, which, however, were all fully compatible with the limits defined by OECD TG 236. This allowed for testing of potential changes in the FET assay due to protocol variations. The standard endpoints of the FET (acute embryotoxicity) did not reveal any acute toxicity for both virgin MPs and MPs spiked with BP3, BaP, and PFOS. With respect to sublethal endpoints, EROD activity was increased after exposure to MPs spiked with BP3 (3 h pulse) and MPs spiked with BaP (96 h continuous exposure). Cyp1a transcription was increased upon exposure to MPs spiked with BP3 or BaP. For the selected combination of MPs particles and contaminants, the basic FET proved not sensitive enough to reveal effects of (virgin and spiked) MPs. However, given that the FET can easily be supplemented by a broad variety of more subtle and sensitive endpoints, an enhanced FET protocol may provide a relevant approach with developmental stages of a vertebrate animal model, which is not protected by current EU animal welfare legislation (Directive EU 2010/63). 
PY 2019
PD SEP
SO Frontiers In Environmental Science
SN 2296-665X
PU Frontiers Media SA
VL 7
IS 135
UT 000486181200001
DI 10.3389/fenvs.2019.00135
ID 62545
ER

EF