FN Archimer Export Format PT J TI Realistic environmental exposure to microplastics does not induce biological effects in the Pacific oyster Crassostrea gigas BT AF Revel, Messika Châtel, Amélie Perrein-Ettajani, Hanane Bruneau, Mélanie Akcha, Farida Sussarellu, Rossana ROUXEL, Julien Costil, Katherine Decottignies, Priscilla Cognie, Bruno Lagarde, Fabienne Mouneyrac, Catherine AS 1:1;2:1;3:1;4:1;5:2;6:2;7:2;8:3;9:4;10:4;11:5;12:1; FF 1:;2:;3:;4:;5:PDG-RBE-BE-LEX;6:PDG-RBE-BE-LEX;7:PDG-RBE-BE-LEX;8:;9:;10:;11:;12:; C1 Laboratoire Mer, Molécules, Santé (MMS EA2160), Université Catholique de l’Ouest, 3 place André Leroy, 49000, Angers, France Ifremer, Laboratoire d’Ecotoxicologie, Rue de l’Ile d’Yeu, BP21105, 44311, Nantes cedex 03, France UMR BOREA, MNHN, UPMC, UCBN, CNRS-7208, IRD-207, SFR ICORE, Université de Caen Normandie, Caen, France FR CNRS 3473 IUML, Laboratoire Mer, Molécules, Santé (MMS EA2160), Université de Nantes, 2 rue de la Houssinière, Nantes Cedex 3, France Institut des Molécules et des Matériaux du Mans, UMR CNRS 6283, Le Mans Université, Avenue Olivier Messiaen, 72085, Le Mans Cedex, France C2 UNIV CATHOLIQUE OUEST, FRANCE IFREMER, FRANCE UNIV CAEN, FRANCE UNIV NANTES, FRANCE UNIV LE MANS, FRANCE SI NANTES SE PDG-RBE-BE-LEX IN WOS Ifremer UPR copubli-france copubli-univ-france IF 1.101 TC 50 UR https://archimer.ifremer.fr/doc/00588/70055/68018.pdf LA English DT Article DE ;Microplastics;Crassostrea gigas;Biodeposits;Biomarkers;Bivalves AB The aim of the present study was to evaluate the presence and potential toxic effects of plastic fragments (<400 μm) of polyethylene and polypropylene on the Pacific oyster Crassostrea gigas. Oysters were exposed to environmentally relevant concentrations (0, 0.008, 10, 100 μg of particles/L) during 10 days, followed by a depuration period of 10 days in clean seawater. Effects of microplastics were evaluated on the clearance rate of organisms, tissue alteration, antioxidant defense, immune alteration and DNA damage. Detection and quantification of microplastics in oyster's tissues (digestive gland, gills and other tissues) and biodeposits using infrared microscopy were also conducted. Microplastics were detected in oyster's biodeposits following exposure to all tested concentrations: 0.003, 0.006 and 0.05 particles/mg of biodeposits in oysters exposed to 0.008, 10 and 100 μg of particles/L, respectively. No significant modulation of biological markers was measured in organisms exposed to microplastics in environmentally relevant conditions. PY 2020 PD JAN SO Marine Pollution Bulletin SN 0025-326X PU Elsevier BV VL 150 UT 000509611200019 DI 10.1016/j.marpolbul.2019.110627 ID 70055 ER EF