FN Archimer Export Format
PT J
TI Genomic Insights into the Carbon and Energy Metabolism of a Thermophilic Deep-Sea Bacterium Deferribacter autotrophicus Revealed New Metabolic Traits in the Phylum Deferribacteres
BT 
AF Slobodkin, Alexander
   Slobodkina, Galina
   Allioux, Maxime
   Alain, Karine
   Jebbar, Mohamed
   Shadrin, Valerian
   Kublanov, Ilya
   Toshchakov, Stepan
   Bonch-Osmolovskaya, Elizaveta
AS 1:1;2:1;3:2;4:3;5:2;6:1;7:1;8:1;9:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;
C1 Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia
   Univ Brest, CNRS, Ifremer, LIaA1211, Laboratoire de Microbiologie des Environnements Extrêmes LM2E, F-29280 Plouzané, France
   Univ Brest, CNRS, Ifremer, LIaA1211, Laboratoire de Microbiologie des Environnements Extrêmes LM2E, F-29280 Plouzané, France
C2 RUSSIAN ACAD SCI, RUSSIA
   UBO, FRANCE
   CNRS, FRANCE
UM BEEP-LM2E
IN WOS Cotutelle UMR
   DOAJ
   copubli-int-hors-europe
IF 1.151
TC 14
UR https://archimer.ifremer.fr/doc/00589/70078/68051.pdf
   https://archimer.ifremer.fr/doc/00589/70078/68052.pptx
   https://archimer.ifremer.fr/doc/00589/70078/68053.xlsx
   https://archimer.ifremer.fr/doc/00589/70078/68054.xlsx
   https://archimer.ifremer.fr/doc/00589/70078/68055.xlsx
   https://archimer.ifremer.fr/doc/00589/70078/68056.xlsx
   https://archimer.ifremer.fr/doc/00589/70078/68057.xlsx
   https://archimer.ifremer.fr/doc/00589/70078/68058.pdf
LA English
DT Article
DE ;autotrophic;thermophile;roTCA cycle;CO oxidation;Fe(III)-reduction;nitrate reduction
AB nformation on the biochemical pathways of carbon and energy metabolism in representatives of the deep lineage bacterial phylum Deferribacteres are scarce. Here, we report the results of the sequencing and analysis of the high-quality draft genome of the thermophilic chemolithoautotrophic anaerobe Deferribacter autotrophicus. Genomic data suggest that CO2 assimilation is carried out by recently proposed reversible tricarboxylic acid cycle (“roTCA cycle”). The predicted genomic ability of D. autotrophicus to grow due to the oxidation of carbon monoxide was experimentally proven. CO oxidation was coupled with the reduction of nitrate to ammonium. Utilization of CO most likely involves anaerobic [Ni, Fe]-containing CO dehydrogenase. This is the first evidence of CO oxidation in the phylum Deferribacteres. The genome of D. autotrophicus encodes a Nap-type complex of nitrate reduction. However, the conversion of produced nitrite to ammonium proceeds via a non-canonical pathway with the participation of hydroxylamine oxidoreductase (Hao) and hydroxylamine reductase. The genome contains 17 genes of putative multiheme c-type cytochromes and “e-pilin” genes, some of which are probably involved in Fe(III) reduction. Genomic analysis indicates that the roTCA cycle of CO2 fixation and putative Hao-enabled ammonification may occur in several members of the phylum Deferribacteres. 
PY 2019
PD NOV
SO Genes
SN 2073-4425
PU MDPI AG
VL 10
IS 11
UT 000502296000015
DI 10.3390/genes10110849
ID 70078
ER

EF