FN Archimer Export Format PT J TI Species-Specific Antioxidant Power and Bioactive Properties of the Extracts Obtained from Wild Mediterranean Calendula Spp. (Asteraceae) BT AF Messina, Concetta Maria Troia, Angelo Arena, Rosaria Manuguerra, Simona Ioannou, Theodora Curcuraci, Eleonora Renda, Giuseppe Hellio, Claire Santulli, Andrea AS 1:1;2:2;3:1;4:1,3;5:;6:1;7:1;8:4;9:1; FF 1:;2:;3:;4:;5:;6:;7:;8:;9:; C1 Laboratorio di Biochimica Marina ed Ecotossicologia, Dipartimento di Scienze della terra e del Mare DiSTeM, Università degli Studi di Palermo, Via G. Barlotta 4, 91100 Trapani, Italy Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), Università degli Studi di Palermo, 90123 Palermo, Italy Department of chemistry, Faculty of Science, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece Univ Brest, CNRS, IRD, Ifremer, LEMAR, Institut Universitaire Européen de la Mer, F-29280 Plouzané, France C2 UNIV PALERMO, ITALY UNIV PALERMO, ITALY UNIV ARISTOTLE THESSALONIKI, GREECE UBO, FRANCE UM LEMAR IN WOS Cotutelle UMR DOAJ copubli-europe IF 1.726 TC 16 UR https://archimer.ifremer.fr/doc/00591/70336/68389.pdf https://archimer.ifremer.fr/doc/00591/70336/68390.pdf LA English DT Article DE ;calendula species;marigold;supercritical fluid extraction;bioactivity;antioxidants;polyphenols AB n this study we focused on four taxa of the genus Calendula (C. maritima, C. suffruticosa subsp. fulgida, C. arvensis, and the hybrid between the first two ones), collected in Mediterranean area (Sicily). Six extracts for each species were obtained using solvents with increasing polarity (hexane, ethanol 80%, acetone 70%, and water) and through extraction by supercritical fluids (SFE). It has been observed that the solvent with the highest extraction efficiency was ethanol 80% for all species. However, SFE extracts showed high antioxidant activity comparable to the ethanol 80% extract (polyphenol, DPPH, and reducing power method). These findings were confirmed by in vitro analysis (MTT assay) where it was observed that the tested concentration (24 µg/mL), obtained from ethanol 80% and SFE extracts, showed a protective effect comparable to that induced by a synthetic antioxidant. Extraction with SFE ensured a great selectivity by avoiding the use of toxic organic solvents and thus consisted of a promising technique for sustainable production of Calendula extracts PY 2019 PD NOV SO Applied Sciences-basel SN 2076-3417 PU MDPI AG VL 9 IS 21 UT 000498058600152 DI 10.3390/app9214627 ID 70336 ER EF