FN Archimer Export Format
PT J
TI Intracellular Infection of Diverse Diatoms by an Evolutionary Distinct Relative of the Fungi
BT 
AF Chambouvet, Aurélie
   Monier, Adam
   Maguire, Finlay
   Itoïz, Sarah
   del Campo, Javier
   Elies, Philippe
   Edvardsen, Bente
   Eikreim, Wenche
   Richards, Thomas A.
AS 1:1;2:2;3:2,3;4:1;5:4;6:5;7:6;8:6;9:2;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;
C1 CNRS, Univ Brest, IRD, Ifremer, LEMAR, F-29280 Plouzane, France
   Living Systems Institute, School of Biosciences, University of Exeter, Exeter, EX4 4QD, UK
   Faculty of Computer Science, Dalhousie University, Halifax, Nova Scotia, B3H 4R2, Canada
   Rosenstiel School of Marine and Atmospheric Science, University of Miami, Miami, FL, 33149 USA
   Plateforme d’Imagerie et de Mesures en Microscopie, Université de Bretagne Occidentale, 29200, Brest, France
   Section for Aquatic Biology and Toxicology, Department of Biosciences, University of Oslo, PO Box 1066 Blindern, 0316 Oslo, Norway
C2 UBO, FRANCE
   UNIV EXETER, UK
   UNIV DALHOUSIE, CANADA
   UNIV MIAMI, USA
   UBO, FRANCE
   UNIV OSLO, NORWAY
UM LEMAR
IN WOS Cotutelle UMR
   copubli-europe
   copubli-int-hors-europe
IF 9.601
TC 17
UR https://archimer.ifremer.fr/doc/00591/70337/68391.pdf
   https://archimer.ifremer.fr/doc/00591/70337/68392.pdf
   https://archimer.ifremer.fr/doc/00591/70337/68393.pdf
LA English
DT Article
AB The Fungi are a diverse kingdom, dominating terrestrial environments and driving important ecologies. Although fungi, and the related Opisthosporidia, interact with photosynthetic organisms on land and in freshwater as parasites, symbionts, and/or saprotrophic degraders [1, 2], such interactions in the marine environment are poorly understood [3, 4, 5, 6, 7, 8]. One newly identified uncultured marine lineage has been named novel chytrid-like-clade-1 (NCLC1) [4] or basal-clone-group-I [5, 6]. We use ribosomal RNA (rRNA) encoding gene phylogenies to demonstrate that NCLC1 is a distinct branch within the Opisthosporidia (Holomycota) [7]. Opisthosporidia are a diverse and largely uncultured group that form a sister branch to the Fungi or, alternatively, the deepest branch within the Fungi, depending on how the boundary to this kingdom is inferred [9]. Using culture-free lineage-specific rRNA-targeted fluorescent in situ hybridization (FISH) microscopy, we demonstrate that NCLC1 cells form intracellular infection of key diatom species, establishing that intracellular colonization of a eukaryotic host is a consistent lifestyle across the Opisthosporidia [8, 9, 10, 11]. NCLC1 infection-associated loss and/or envelopment of the diatom nuclei infers a necrotrophic-pathogenic interaction. Diatoms are one of the most diverse and ecologically important phytoplankton groups, acting as dominant primary producers and driving carbon fixation and storage in many aquatic environments [12, 13, 14]. Our results provide insight into the diversity of microbial eukaryotes that interact with diatoms. We suggest that such interactions can play a key role in diatom associated ecosystem functions, such as the marine carbon pump through necrotrophic-parasitism, facilitating the export of diatoms to the sediment [15, 16]. 
PY 2019
PD DEC
SO Current Biology
SN 0960-9822
PU Elsevier BV
VL 29
IS 23
UT 000499948700030
DI 10.1016/j.cub.2019.09.074
ID 70337
ER

EF