Characterization of biofilm extracts from two marine bacteria

Type Article
Date 2019-11
Language English
Author(s) Passerini DelphineORCID1, Fecamp Florian1, Marchand Laetitia1, Kolypczuk LaetitiaORCID1, Bonnetot Sandrine1, Sinquin CorinneORCID1, Verrez-Bagnis VeroniqueORCID1, Hervio Heath DominiqueORCID2, Colliec Jouault SylviaORCID1, Delbarre Ladrat ChristineORCID1
Affiliation(s) 1 : Ifremer, Atlantique Center, Microbial Ecosystems and Marine Molecules for the Biotechnologies, 44311 Rue de l’Ile d’Yeu, Nantes CEDEX 3, France
2 : Ifremer, Bretagne Center, Health, Environment and Microbiology, 1625 Route de Sainte-Anne, 29280 Plouzané, France
Source Applied Sciences-basel (2076-3417) (MDPI), 2019-11 , Vol. 9 , N. 22 , P. 4971 (20p.)
DOI 10.3390/app9224971
WOS© Times Cited 4
Note This article belongs to the Special Issue Polysaccharides from Marine Environments
Keyword(s) Vibrio, biofilm, polysaccharide, quorum-sensing, activity

In the marine environment, biofilm formation is an important lifestyle for microorganisms. A biofilm is comprised of cells embedded in an extracellular matrix that holds them close together and keeps the biofilm attached to the colonized surface. This predominant lifestyle and its main regulation pathway, namely quorum-sensing (QS), have been shown to induce specific bioactive metabolites. In this study, we investigated the biofilm formation by two marine bacteria belonging to the Vibrio species to discover potentially innovative bioactive compounds. We proposed a protocol to isolate biofilm extracts, to analyze their biochemical composition, and to compare them to planktonic cell extracts. Cells were grown attached to a plastic surface; extracts were prepared in water, NaOH, or in ethyl acetate and analyzed. Extracellular matrix components featured carbohydrates, proteins, lipids, and low amount of DNA. Carbohydrates appeared to be the main constituent of biofilm but also of the planktonic cell supernatant. Moreover, antimicrobial and QS-signaling activities were evidenced in extracts.

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Passerini Delphine, Fecamp Florian, Marchand Laetitia, Kolypczuk Laetitia, Bonnetot Sandrine, Sinquin Corinne, Verrez-Bagnis Veronique, Hervio Heath Dominique, Colliec Jouault Sylvia, Delbarre Ladrat Christine (2019). Characterization of biofilm extracts from two marine bacteria. Applied Sciences-basel, 9(22), 4971 (20p.). Publisher's official version : , Open Access version :