FN Archimer Export Format
PT J
TI Characterization of biofilm extracts from two marine bacteria
BT 
AF PASSERINI, Delphine
   FECAMP, Florian
   MARCHAND, Laetitia
   KOLYPCZUK, Laetitia
   BONNETOT, Sandrine
   SINQUIN, Corinne
   VERREZ-BAGNIS, Veronique
   HERVIO HEATH, Dominique
   COLLIEC JOUAULT, Sylvia
   DELBARRE LADRAT, Christine
AS 1:1;2:1;3:1;4:1;5:1;6:1;7:1;8:2;9:1;10:1;
FF 1:PDG-RBE-BRM-LEMMMB;2:;3:PDG-RBE-BRM-LEMMMB;4:PDG-RBE-BRM-LEMMMB;5:PDG-RBE-BRM-LEMMMB;6:PDG-RBE-BRM-LEMMMB;7:PDG-RBE-BRM-LEMMMB;8:PDG-RBE-SGMM-LSEM;9:PDG-RBE-BRM-LEMMMB;10:PDG-RBE-BRM-LEMMMB;
C1 Ifremer, Atlantique Center, Microbial Ecosystems and Marine Molecules for the Biotechnologies, 44311 Rue de l’Ile d’Yeu, Nantes CEDEX 3, France
   Ifremer, Bretagne Center, Health, Environment and Microbiology, 1625 Route de Sainte-Anne, 29280 Plouzané, France
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI NANTES
   BREST
SE PDG-RBE-BRM-LEMMMB
   PDG-RBE-SGMM-LSEM
IN WOS Ifremer UPR
   DOAJ
IF 1.726
TC 4
UR https://archimer.ifremer.fr/doc/00592/70381/68461.pdf
   https://archimer.ifremer.fr/doc/00592/70381/68488.pdf
LA English
DT Article
CR HERO91
   MICROSMOKE
BO Le Nadir
DE ;Vibrio;biofilm;polysaccharide;quorum-sensing;activity
AB In the marine environment, biofilm formation is an important lifestyle for microorganisms. A biofilm is comprised of cells embedded in an extracellular matrix that holds them close together and keeps the biofilm attached to the colonized surface. This predominant lifestyle and its main regulation pathway, namely quorum-sensing (QS), have been shown to induce specific bioactive metabolites. In this study, we investigated the biofilm formation by two marine bacteria belonging to the Vibrio species to discover potentially innovative bioactive compounds. We proposed a protocol to isolate biofilm extracts, to analyze their biochemical composition, and to compare them to planktonic cell extracts. Cells were grown attached to a plastic surface; extracts were prepared in water, NaOH, or in ethyl acetate and analyzed. Extracellular matrix components featured carbohydrates, proteins, lipids, and low amount of DNA. Carbohydrates appeared to be the main constituent of biofilm but also of the planktonic cell supernatant. Moreover, antimicrobial and QS-signaling activities were evidenced in extracts. 
PY 2019
PD NOV
SO Applied Sciences-basel
SN 2076-3417
PU MDPI
VL 9
IS 22
UT 000502570800248
DI 10.3390/app9224971
ID 70381
ER

EF