FN Archimer Export Format
PT J
TI Is pallial mucus involved in Ostrea edulis defenses against the parasite Bonamia ostreae?
BT 
AF Fernández-Boo, Sergio
   Gervais, Ophelie
   Prado-Alvarez, Maria
   Chollet, Bruno
   Claverol, Stéphane
   Lecadet, Cyrielle
   Dubreuil, Christine
   Arzul, Isabelle
AS 1:1,4;2:1;3:3;4:1;5:2;6:1;7:1;8:1;
FF 1:PDG-RBE-SG2M-LGPMM;2:PDG-RBE-SGMM-LGPMM;3:;4:PDG-RBE-SGMM-LGPMM;5:;6:PDG-RBE-SGMM-LGPMM;7:PDG-RBE-SGMM-LGPMM;8:PDG-RBE-SGMM-LGPMM;
C1 Institut Français de Recherche pour ĺExploitation de la Mer (IFREMER), Laboratoire de Génétique et Pathologie (LGP), Avenue Mus de Loup, 17390 La Tremblade, France
   Université de Bordeaux, Centre Génomique Fonctionnelle de Bordeaux, Plateforme Protéome, F-33000 Bordeaux, France
   Aquatic Molecular Pathobiology Group, Marine Research Institute (IIM-CSIC). Vigo, Spain
   Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), University of Porto, Avenida General Norton de Matos, S/N, 4450-208 Matosinhos, Portugal
C2 IFREMER, FRANCE
   UNIV BORDEAUX, FRANCE
   IIM CSIC, SPAIN
   CIIMAR, PORTUGAL
SI LA TREMBLADE
SE PDG-RBE-SG2M-LGPMM
   PDG-RBE-SGMM-LGPMM
IN WOS Ifremer UPR
   copubli-france
   copubli-europe
   copubli-univ-france
IF 2.841
TC 11
UR https://archimer.ifremer.fr/doc/00594/70602/68795.pdf
LA English
DT Article
DE ;Bonamia ostreae;Crassostrea gigas;Ostrea edulis;Flow cytometry;Immune response;Proteome
AB Bonamia ostreae is an intrahemocytic parasite that has been responsible for severe mortalities in the flat oyster Ostrea edulis since the 1970́s. The Pacific oyster Crassostrea gigas is considered to be resistant to the disease and appears to have mechanisms to avoid infection. Most studies carried out on the invertebrate immune system focus on the role of hemolymph, although mucus, which covers the body surface of molluscs, could also act as a barrier against pathogens. In this study, the in vitro effect of mucus from the oyster species Ostrea edulis and C. gigas on B. ostreae was investigated using flow cytometry. Results showed an increase in esterase activities and mortality rate of parasites exposed to mucus from both oyster species. In order to better understand the potential role of mucus in the defense of the oyster against parasites such as B. ostreae, liquid chromatography and tandem mass spectrometry were used to describe and compare mucus protein composition from both species. In all oyster species, pallial mucus contains a high level of proteins; however, O. edulis mucus produced a variety of proteins that could be involved in the immune response against the parasite, including Cu/Zn extracellular superoxide dismutase, thioxiredoxin, peroxiredon VI, heat shock protein 90 as well as several hydrolases. Conversely, a different set of antioxidant proteins, hydrolases and stress related proteins were identified in mucus from C. gigas. Our results suggest an innate immunity adaptation of oysters to develop a specific response against their respective pathogens. The mucosal protein composition also provides new insights for further investigations into the immune response in oysters. 
PY 2020
PD JAN
SO Journal Of Invertebrate Pathology
SN 0022-2011
PU Elsevier BV
VL 169
UT 000506721300011
DI 10.1016/j.jip.2019.107259
ID 70602
ER

EF