FN Archimer Export Format
PT J
TI Potential of fascaplysin and palauolide from Fascaplysinopsis cf reticulata to reduce the risk of bacterial infection in fish farming
BT 
AF Mai, Tepoerau
   Toullec, Jordan
   Van Wynsberge, Simon
   Besson, Marc
   Soulet, Stephanie
   Petek, Sylvain
   Aliotti, Emmanuelle
   Ekins, Merrick
   Hall, Kathryn
   Erpenbeck, Dirk
   Lecchini, David
   Beniddir, Mehdi A.
   SAULNIER, Denis
   Debitus, Cecile
AS 1:1;2:11;3:1,3,4;4:5,6;5:12;6:13;7:13;8:7,8;9:7;10:9;11:5;12:10;13:1;14:13;
FF 1:PDG-RBE-LEADNC;2:;3:PDG-RBE-LEADNC;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:PDG-RBE-RMPF;14:;
C1 EIO, ILM, IFREMER, IRD, UPF, BP 6570, 98702 FAA’A, Tahiti, French Polynesia
   IRD, Univ. Brest, CNRS, IFREMER, LEMAR, F-29280 Plouzane, France
   Institut de Recherche pour le Développement, UMR 9220 ENTROPIE (Institut de Recherche Pour le Développement, Université de la Réunion, Centre National de la Recherche Scientifique), Nouméa, New Caledonia
   LEAD-NC, 98848 Nouméa, New Caledonia
   PSL Research University: EPHE-UPVD-CNRS, USR 3278 CRIOBE, BP 1013-Moorea, French Polynesia
   Observatoire Océanologique de Banyuls-sur-Mer, Université Pierre et Marie Curie Paris, Banyuls-sur-Mer, France
   Queensland Museum, PO Box 3300, South Brisbane BC, Queensland 4101, Australia
   School of Biological Sciences, University of Queensland, St Lucia, Queensland, 4072, Australia
   Department of Earth and Environmental Sciences & GeoBio-Center, Ludwig-Maximilians-Universität München, Richard-Wagner Str. 10, 80333 Munich, Germany
   Biocis, Equipe “Pharmacognosie-Chimie des substances naturelles”, CNRS, Université de Paris Sus, Univ. Paris-Saclay, Faculté de pharmacie, 5 rue J-B Clément, 92293 Châtenay-Malabry cedex, France
   EIO, ILM, IFREMER, IRD, UPF, BP 6570, 98702 FAA’A, Tahiti, French Polynesia
   EIO, ILM, IFREMER, IRD, UPF, BP 6570, 98702 FAA’A, Tahiti, French Polynesia
   EIO, ILM, IFREMER, IRD, UPF, BP 6570, 98702 FAA’A, Tahiti, French Polynesia
C2 IFREMER, FRANCE
   IRD, FRANCE
   IRD, FRANCE
   IFREMER, FRANCE
   EPHE, FRANCE
   UNIV PARIS 06, FRANCE
   QUEENSLAND MUSEUM, AUSTRALIA
   UNIV QUEENSLAND, AUSTRALIA
   UNIV MUNCHEN, GERMANY
   UNIV PARIS SACLAY, FRANCE
   UBO, FRANCE
   UNIV POLYNESIE FRANCAISE, FRANCE
   IRD, FRANCE
SI NOUMEA
   BREST
   TAHITI
SE PDG-RBE-LEADNC
   PDG-RBE-PFOM
   IRD
   PDG-RBE-RMPF
UM LEMAR
   EIO
IN DOAJ
TC 0
UR https://archimer.ifremer.fr/doc/00598/71052/69366.pdf
LA English
DT Article
CR TUAM 2011
BO Alis
DE ;Porifera;Marine natural products;Quorum sensing inhibitors;Antibiotic;Fascaplysinopsis cf reticulata
AB Marine natural products isolated from the sponge Fascaplysinopsis cf reticulata, in French Polynesia, were investigated as an alternative to antibiotics to control pathogens in aquaculture. The overuse of antibiotics in aquaculture is largely considered to be an environmental pollution, because it supports the transfer of antibiotic resistance genes within the aquatic environment. One environmentally friendly alternative to antibiotics is the use of quorum sensing inhibitors (QSIs). Quorum sensing (QS) is a regulatory mechanism in bacteria which control virulence factors through the secretion of autoinducers (AIs), such as acyl-homoserine lactone (AHL) in gram-negative bacteria. Vibrio harveyi QS is controlled through three parallel pathways: HAI-1, AI-2, and CAI-1. Bioassay-guided purification of F. cf reticulata extract was conducted on two bacterial species, i.e., Tenacibaculum maritimum and V. harveyi for antibiotic and QS inhibition bioactivities. Toxicity bioassay of fractions was also evaluated on the freshwater fish Poecilia reticulata and the marine fish Acanthurus triostegus. Cyclohexanic and dichloromethane fractions of F. cf reticulata exhibited QS inhibition on V. harveyi and antibiotic bioactivities on V. harveyi and T. maritimum, respectively. Palauolide (1) and fascaplysin (2) were purified as major molecules from the cyclohexanic and dichloromethane fractions, respectively. Palauolide inhibited QS of V. harveyi through HAI-1 QS pathway at 50 μg ml–1 (26 μM), while fascaplysin affected the bacterial growth of V. harveyi (50 μg ml–1) and T. maritimum (0.25 μg). The toxicity of fascaplysin-enriched fraction (FEF) was evaluated and exhibited a toxic effect against fish at 50 μg ml–1. This study demonstrated for the first time the QSI potential of palauolide (1). Future research may assess the toxicity of both the cyclohexanic fraction of the sponge and palauolide (1) on fish, to confirm their potential as alternative to antibiotics in fish farming. 
PY 2019
PD DEC
SO Fisheries and Aquatic Sciences
SN 2234-1757
PU Springer Science and Business Media LLC
VL 22
IS 1
DI 10.1186/s41240-019-0145-0
ID 71052
ER

EF