FN Archimer Export Format
PT J
TI Supplementation of live yeast based feed additive in early life promotes rumen microbial colonization and fibrolytic potential in lambs
BT 
AF CHAUCHEYRAS-DURAND, Frederique
   AMEILBONNE, Aurelie
   AUFFRET, Pauline
   BERNARD, Mickael
   MIALON, Marie-Madeleine
   DUNIERE, Lysiane
   FORANO, Evelyne
AS 1:1,2;2:1,2;3:2;4:3;5:4;6:1,2;7:2;
FF 1:;2:;3:;4:;5:;6:;7:;
C1 Lallemand SAS, F-31702 Blagnac, France.
   Univ Clermont Auvergne, INRA, UMR 454, MEDIS, F-63000 Clermont Ferrand, France.
   INRA Auvergne Rhone Alpes, UE 1414, Herbipole, St Genes Champanelle, France.
   Univ Clermont Auvergne, INRA, VetAgro Sup, Herbivores,UMR 1213, F-63000 Clermont Ferrand, France.
C2 LALLEMAND SAS, FRANCE
   UNIV CLERMONT AUVERGNE, FRANCE
   INRA, FRANCE
   UNIV CLERMONT AUVERGNE, FRANCE
IN DOAJ
IF 3.998
TC 26
UR https://archimer.ifremer.fr/doc/00599/71077/69395.pdf
   https://archimer.ifremer.fr/doc/00599/71077/69396.pdf
LA English
DT Article
AB Rumen microbiota is of paramount importance for ruminant digestion efficiency as the microbial fermentations supply the host animal with essential sources of energy and nitrogen. Early separation of newborns from the dam and distribution of artificial milk (Artificial Milking System or AMS) could impair rumen microbial colonization, which would not only affect rumen function but also have possible negative effects on hindgut homeostasis, and impact animal health and performance. In this study, we monitored microbial communities in the rumen and the feces of 16 lambs separated from their dams from 12 h of age and artificially fed with milk replacer and starter feed from d8, in absence or presence of a combination of the live yeast Saccharomyces cerevisiae CNCM I-1077 and selected yeast metabolites. Microbial groups and targeted bacterial species were quantified by qPCR and microbial diversity and composition were assessed by 16S rDNA amplicon sequencing in samples collected from birth to 2 months of age. The fibrolytic potential of the rumen microbiota was analyzed with a DNA microarray targeting genes coding for 8 glycoside hydrolase (GH) families. In Control lambs, poor establishment of fibrolytic communities was observed. Microbial composition shifted as the lambs aged. The live yeast supplement induced significant changes in relative abundances of a few bacterial OTUs across time in the rumen samples, among which some involved in crucial rumen function, and favored establishment of Trichostomatia and Neocallimastigaceae eukaryotic families. The supplemented lambs also harbored greater abundances in Fibrobacter succinogenes after weaning. Microarray data indicated that key cellulase and hemicellulase encoding-genes were present from early age in the rumen and that in the Supplemented lambs, a greater proportion of hemicellulase genes was present. Moreover, a higher proportion of GH genes from ciliate protozoa and fungi was found in the rumen of those animals. This yeast combination improved microbial colonization in the maturing rumen, with a potentially more specialized ecosystem towards efficient fiber degradation, which suggests a possible positive impact on lamb gut development and digestive efficiency. 
PY 2019
PD DEC
SO Scientific Reports
SN 2045-2322
PU Nature Publishing Group
VL 9
UT 000503190400005
DI 10.1038/s41598-019-55825-0
ID 71077
ER

EF