FN Archimer Export Format
PT J
TI Intraspecific Variability in the Toxin Production and Toxin Profiles of In Vitro Cultures of Gambierdiscus polynesiensis (Dinophyceae) from French Polynesia
BT 
AF Longo, Sebastien
   Sibat, Manoella
   Viallon, Jérôme
   Darius, Hélène Taiana
   Hess, Philipp
   Chinain, Mireille
AS 1:1,2;2:2;3:1;4:1;5:2;6:1;
FF 1:;2:PDG-ODE-DYNECO-PHYC;3:;4:;5:PDG-ODE-DYNECO-PHYC;6:;
C1 Laboratoire de recherche sur les Biotoxines Marines Institut Louis Malardé-UMR 241 EIO, 98713 Papeete-Tahiti, French Polynesia
   Laboratoire Phycotoxines, IFREMER, Rue de l’Ile d’Yeu, 44311 Nantes, France
C2 INST LOUIS MALARDE, FRANCE
   IFREMER, FRANCE
SI NANTES
SE PDG-ODE-DYNECO-PHYC
UM EIO
IN WOS Ifremer UPR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
IF 2.13
TC 38
UR https://archimer.ifremer.fr/doc/00599/71134/69449.pdf
LA English
DT Article
DE ;Gambierdiscus polynesiensis;ciguatera;ciguatoxins;gambierone;44-methylgambierone;LC-MS/MS;CBA-N2a;toxin profiles
AB Ciguatera poisoning (CP) is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. The toxin production and toxin profiles were explored in four clones of G. polynesiensis originating from different islands in French Polynesia with contrasted CP risk: RIK7 (Mangareva, Gambier), NHA4 (Nuku Hiva, Marquesas), RAI-1 (Raivavae, Australes), and RG92 (Rangiroa, Tuamotu). Productions of CTXs, maitotoxins (MTXs), and gambierone group analogs were examined at exponential and stationary growth phases using the neuroblastoma cell-based assay and liquid chromatography–tandem mass spectrometry. While none of the strains was found to produce known MTX compounds, all strains showed high overall P-CTX production ranging from 1.1 ± 0.1 to 4.6 ± 0.7 pg cell−1. In total, nine P-CTX analogs were detected, depending on strain and growth phase. The production of gambierone, as well as 44-methylgamberione, was also confirmed in G. polynesiensis. This study highlighted: (i) intraspecific variations in toxin production and profiles between clones from distinct geographic origins and (ii) the noticeable increase in toxin production of both CTXs, in particular CTX4A/B, and gambierone group analogs from the exponential to the stationary phase 
PY 2019
PD DEC
SO Toxins
SN 2072-6651
PU MDPI AG
VL 11
IS 12
UT 000507337800058
DI 10.3390/toxins11120735
ID 71134
ER

EF