FN Archimer Export Format
PT J
TI Exploring Environmental DNA (eDNA) to Assess Biodiversity of Hard Substratum Faunal Communities on the Lucky Strike Vent Field (Mid-Atlantic Ridge) and Investigate Recolonization Dynamics After an Induced Disturbance
BT 
AF Cowart, Dominique
   MATABOS, Marjolaine
   Brandt, Miriam
   Marticorena, Julien
   Sarrazin, Jozee
AS 1:1;2:1;3:2;4:1;5:1;
FF 1:PDG-REM-EEP-LEP;2:PDG-REM-EEP-LEP;3:PDG-RBE-MARBEC-LHM;4:PDG-REM-EEP-LEP;5:PDG-REM-EEP-LEP;
C1 Ifremer Centre de Bretagne, REM/EEP, Laboratoire Environnement Profond, Plouzané, France
   IRD, CNRS, MARBEC, Ifremer, Université de Montpellier, Sète, France
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI BREST
   SETE
SE PDG-REM-EEP-LEP
   PDG-RBE-MARBEC-LHM
UM MARBEC
IN WOS Ifremer UPR
   WOS Ifremer UMR
   DOAJ
IF 5.247
TC 15
UR https://archimer.ifremer.fr/doc/00600/71176/69515.pdf
   https://archimer.ifremer.fr/doc/00600/71176/69516.pdf
   https://archimer.ifremer.fr/doc/00600/71176/69517.pdf
   https://archimer.ifremer.fr/doc/00600/71176/69518.tif
   https://archimer.ifremer.fr/doc/00600/71176/69519.tif
   https://archimer.ifremer.fr/doc/00600/71176/69520.tif
   https://archimer.ifremer.fr/doc/00600/71176/69521.tif
   https://archimer.ifremer.fr/doc/00600/71176/69522.tif
   https://archimer.ifremer.fr/doc/00600/71176/69523.tif
   https://archimer.ifremer.fr/doc/00600/71176/69524.tif
   https://archimer.ifremer.fr/doc/00600/71176/69525.tif
LA English
DT Article
CR MOMARSAT : MONITORING THE MID ATLANTIC RIDGE
   MOMARSAT2017
   MOMARSAT2018
BO Pourquoi pas ?
   L'Atalante
DE ;natural regeneration;clearance;Bathymodiolus azoricus;monitoring;hard substratum;active and inactive vent sites
AB Deep ocean hydrothermal vent ecosystems face physical disturbances from naturally occurring volcanic and tectonic activities and are at increasing risk of mineral resource exploitation, raising concerns about the resilience of endemic biological communities. Following destructive events, efficient and rapidly applicable surveys of organisms are required to monitor the state, evolution and a possible return of these ecosystems to their original baseline status. In this study, we explored the environmental DNA (eDNA) approach as a tool (1) to assess biodiversity of benthic communities associated with deep-sea hard substrata and (2) tracked the recolonization dynamics of benthic invertebrate communities living on the Montségur edifice within the Lucky Strike vent field (Mid-Atlantic Ridge), after an induced disturbance that consisted of faunal clearance within experimental quadrats. Hard substratum samples were collected prior to and one year after the disturbance, for eDNA metabarcoding using one marker of the mitochondrial Cytochrome Oxidase I (COI) gene and three markers of the nuclear 18S ribosomal RNA (18S) gene. We also generated a DNA barcoding inventory that consisted of taxa physically collected from Montségur and morphologically identified. This inventory contained amplified barcodes from COI, 18S and the nuclear large subunit ribosomal RNA (28S) gene. The resulting sequence information from the COI and 18S were used for eDNA taxonomic assignment. The eDNA datasets uncovered a high diversity of metazoan OTUs, which included macro- and meiofauna common to Lucky Strike. Baseline data collected at the start of the experiment identified higher OTU richness at sites peripheral to the active edifice, as well as at inactive sites. One year following the initial disturbance, analysis of recolonization data found no statistical difference in presence/absence from baseline communities. The eDNA protocols provide a reproducible strategy to quickly assess biodiversity associated with deep sea hard substratum, enabling comparisons across various habitats. To follow recolonization dynamics at small spatial scales, however, we recommend an approach that uses both molecular and morphological-based traditional methods. Finally, we present original data on the “unseen” diversity of the fauna inhabiting the poorly studied inactive sites, locations that are targeted by commercial mining. Continued monitoring of these sites is currently ongoing and will bring new insight on recovery potential over time, with the ultimate goal of informing conservation and management decisions in relation to the protection of hydrothermal vent ecosystems. 
PY 2020
PD JAN
SO Frontiers In Marine Science
SN 2296-7745
PU Frontiers Media SA
VL 6
IS 783
UT 000619937700001
DI 10.3389/fmars.2019.00783
ID 71176
ER

EF