FN Archimer Export Format PT J TI Photochemistry on the Space Station-Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station BT AF COUSSOT, Gaelle LE POSTOLLEC, Aurelie INCERTI, Sebastien BAQUE, Mickael FAYE, Clement VANDENABEELE TRAMBOUZE, Odile COTTIN, Herve RAVELET, Corinne PEYRIN, Eric FIORE, Emmanuelle VIGIER, Flavie CARON, Jerome CHAPUT, Didier PRZYBYLA, Bartos BERGER, Thomas DOBRIJEVIC, Michel AS 1:1;2:2;3:3;4:4;5:5;6:6;7:7,8;8:9;9:9;10:9;11:;12:10;13:11;14:12;15:12;16:2; FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;14:;15:;16:; C1 Univ Montpellier, ENSCM, CNRS, IBMM, F-34093 Montpellier 5, France. Univ Bordeaux, B18N, CNRS, LAB, Pessac, France. Univ Bordeaux, UMR 5797, CENBG, Gradignan, France. German Aerosp Ctr DLR, Inst Planetary Res Management & Infrastruct, Res Grp Astrobiol Labs, Berlin, Germany. Colcom, Cap Alpha, Clapiers, France. UBO, LMEE, IUEM UMR 6197, Plouzane, France. Univ Paris Est Creteil, UMR 7583, LISA, Creteil, France. Univ Paris Diderot, Inst Pierre Simon Lapl, Creteil, France. Univ Grenoble Alpes, Dept Pharmacochim Mol, CNRS, UMR 5063, St Martin Dheres, France. Comprehens Canc Ctr, Inst Bergonie, Dept Radiotherapie, Bordeaux, France. Ctr Natl Etud Spatiales, DCT ME EM, Toulouse, France. German Aerosp Ctr, Inst Aerosp Med, Cologne, Germany. C2 UNIV MONTPELLIER, FRANCE UNIV BORDEAUX, FRANCE UNIV BORDEAUX, FRANCE GERMAN AEROSP CTR, GERMANY COLCOM, FRANCE UBO, FRANCE UNIV UPEC, FRANCE UNIV PARIS 07, FRANCE UNIV GRENOBLE ALPES, FRANCE COMPREHENS CANC CTR, FRANCE CNES, FRANCE GERMAN AEROSP CTR, GERMANY UM BEEP-LM2E IN WOS Cotutelle UMR copubli-france copubli-europe copubli-univ-france IF 4.091 TC 6 UR https://archimer.ifremer.fr/doc/00601/71306/69723.pdf LA English DT Article DE ;Astrobiology;Cosmic rays;Biochip;Aptamers AB Some microarray-based instruments that use bioaffinity receptors such as antibodies or aptamers are under development to detect signatures of past or present life on planetary bodies. Studying the resistance of such instruments against space constraints and cosmic rays in particular is a prerequisite. We used several ground-based facilities to study the resistance of aptamers to various types of particles (protons, electrons, neutrons, and carbon ions) at different energies and fluences. We also tested the resistance of aptamers during the EXPOSE-R2 mission outside the International Space Station (ISS). The accumulated dose measured after the 588 days of this mission (220 mGy) corresponds to the accumulated dose that can be expected during a mission to Mars. We found that the recognition ability of fluorescently labeled aptamers was not significantly affected during short-term exposure experiments taking into account only one type of radiation at a time. However, we demonstrated that the same fluorescent dye was significantly affected by temperature variations (-21 degrees C to +58 degrees C) and storage throughout the entirety of the ISS experiment (60% of signal loss). This induced a large variability of aptamer signal in our analysis. However, we found that >50% of aptamers were still functional after the whole EXPOSE-R2 mission. We conclude that aptamer-based instruments are well suited for in situ analysis on planetary bodies, but the detection step requires additional investigations. PY 2019 PD AUG SO Astrobiology SN 1531-1074 PU Mary Ann Liebert, Inc VL 19 IS 8 UT 000478581200009 BP 1063 EP 1074 DI 10.1089/ast.2018.1896 ID 71306 ER EF