FN Archimer Export Format
PT J
TI Osmoregulatory performance and immunolocalization of Na+/K+-ATPase in the branchiopod Artemia salina from the Sebkha of Sidi El Hani (Tunisia)
BT 
AF Sellami, Imene
   Charmantier, Guy
   Naceur, Hachem B.
   Kacem, Adnane
   Lorin-Nebel, Catherine
AS 1:1,2;2:1;3:2;4:2;5:2;
FF 1:;2:;3:;4:;5:;
C1 Univ. Montpellier, UMR MARBEC (CNRS, IFREMER, IRD, UM), France
   LR14ES06 Bioressources, Integrative Biology and Valorization, Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Tahar Hadded, BP 74, 5000 Monastir, Tunisia
C2 UNIV MONTPELLIER, FRANCE
   UNIV MONASTIR, TUNISIA
UM MARBEC
IN WOS Cotutelle UMR
   copubli-int-hors-europe
   copubli-sud
IF 2.466
TC 2
UR https://archimer.ifremer.fr/doc/00602/71426/69911.pdf
LA English
DT Article
DE ;Artemia salina;Hypo-osmoregulation;Na+/K+ -ATPase;Immunolocalization;Metepipodites;Maxillary glands
AB Artemia salina is an extremophile species that tolerates a wide range of salinity, especially hypertonic media considered lethal for the majority of other aquatic species. In this study, A. salina cysts were hatched in the laboratory and nauplii were acclimated at three different salinities (60, 139 and 212 ppt). Once in the adult phase, their hemolymph osmolality was measured. The animals were strong hypo-osmoregulators in the entire range of tested salinities, with up to 10 fold lower hemolymph osmolalities than their surrounding environment. Immunostaining of Na+/K+-ATPase was done on sections and on whole body mounts of adults in order to localize the ionocytes in different organs. An intense Na+/K+-ATPase immunostaining throughout the cells was observed in the epithelium of the ten pairs of metepipodites. A positive immunoreactivity for Na+/K+-ATPase was also detected in the maxillary glands, in the epithelium of the efferent tubule and of the excretory canal, as well as in the anterior digestive tract. This study confirms the strong hypo-osmotic capacity of this species and affords an overview of the different organs involved in osmoregulation in A. salina adults. Abbreviations. ADT: anterior digestive tract; Cs: coelomic sac; E: eye; EC: excretory canal; Me: metepipodite; MG: maxillary gland; N: nucleus; Ph: phyllopod; PI: posterior intestine; T: tubule 
PY 2020
PD APR
SO Tissue & Cell
SN 0040-8166
PU Elsevier BV
VL 63
UT 000522853900012
DI 10.1016/j.tice.2020.101340
ID 71426
ER

EF