FN Archimer Export Format PT J TI Ostreopsis cf. ovata (Dinophyceae) Molecular Phylogeny, Morphology, and Detection of Ovatoxins in Strains and Field Samples from Brazil BT AF Nascimento, Silvia M Neves, Raquel AF DE'CARLI, Gabriela AL BORSATO, Geovanna T DA SILVA, Rodrigo AF MELO, Guilherme A DE MORAIS, Agatha M Cockell, Thais C FRAGA, Santiago Menezes-Salgueiro, Adriana D Mafra, Luis L Jr HESS, Philipp Salgueiro, Fabiano AS 1:1;2:1;3:1;4:1;5:1;6:2;7:1;8:1;9:3;10:4;11:5;12:6;13:2; FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:PDG-ODE-DYNECO-PHYC;13:; C1 Laboratório de Microalgas Marinhas, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Av. Pasteur, 458, 314-B, Rio de Janeiro, RJ 22290-240, Brazil Laboratório de Biodiversidade e Evolução Molecular, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Av. Pasteur, 458, 512, Rio de Janeiro, RJ 22290-240, Brazil Centro Oceanográfico de Vigo, Instituto Español de Oceanografía (IEO), Subida a Radio Faro 50, 36390 Vigo, Spain Laboratório de Biotecnologia Vegetal, Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro, Rua Senador Furtado, 121, 112. Maracanã, Rio de Janeiro, RJ 20270-021, Brazil Centro de Estudos do Mar, Universidade Federal do Paraná, Cx. Postal 61, Pontal do Paraná, PR 83255-976, Brazil IFREMER, Laboratoire Phycotoxines, Rue de l’Ile d’Yeu, 44311 Nantes, France C2 UNIV FED RIO DE JANEIRO, BRAZIL UNIV FED RIO DE JANEIRO, BRAZIL IEO, SPAIN INST FED RIO JANEIRO, BRAZIL UNIV FED PARANA, BRAZIL IFREMER, FRANCE SI NANTES SE PDG-ODE-DYNECO-PHYC IN WOS Ifremer UPR DOAJ copubli-europe copubli-int-hors-europe copubli-sud IF 2.13 TC 17 UR https://archimer.ifremer.fr/doc/00604/71594/70026.pdf https://archimer.ifremer.fr/doc/00604/71594/70027.pdf LA English DT Article DE ;benthic dinoflagellates;harmful algal blooms;ovatoxins;taxonomy;morphometry AB Recurrent blooms of Ostreopsis cf. ovata have been reported in Brazil and the Mediterranean Sea with associated ecological, and in the latter case, health impacts. Molecular data based on the D1–D3 and D8–D10 regions of the LSU rDNA and ITS loci, and the morphology of O. cf. ovata isolates and field populations from locations along the Brazilian tropical and subtropical coastal regions and three oceanic islands are presented. Additional ITS sequences from three single cells from the tropical coast are provided. Toxin profiles and quantities of PLTX and their analogues; OVTXs; contained in cells from two clonal cultures and two field blooms from Rio de Janeiro were investigated. Morphology was examined using both light and epifluorescence microscopy. Morphometric analysis of different strains and field populations from diverse locations were compared. Molecular analysis showed that six of the seven sequences grouped at the large “Atlantic/Mediterranean/Pacific” sub-clade, while one sequence branched in a sister clade with sequences from Madeira Island and Greece. The toxin profile of strains and bloom field samples from Rio de Janeiro were dominated by OVTX-a and -b, with total cell quotas (31.3 and 39.3 pg cell−1) in the range of that previously reported for strains of O. cf. ovata. PY 2020 PD FEB SO Toxins SN 2072-6651 PU MDPI VL 12 IS 2 UT 000519117300079 DI 10.3390/toxins12020070 ID 71594 ER EF