FN Archimer Export Format
PT J
TI Quantification of light Polycyclic Aromatic Hydrocarbons in seafood samples using on-line Dynamic HeadSpace extraction, ThermoDesorption, Gas Chromatography tandem Mass Spectrometry, based on an isotope dilution approach
BT 
AF GUIFFARD, Ingrid
   GENY, Thomas
   VEYRAND, Bruno
   MARCHAN, Philippe
   GROUHEL-PELLOUIN, Anne
   BIZEC, Bruno LE
   BICHON, Emmanuelle
AS 1:1;2:1;3:1;4:1;5:2;6:1;7:1;
FF 1:;2:;3:;4:;5:PDG-RBE-BE;6:;7:;
C1 LABERCA, Oniris, INRA, Route de Gachet, CS50707, 44307 NANTES cedex 3, FRANCE
   IFREMER, Laboratory of Biogeochemistry and ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, 44311 NANTES cedex 3, FRANCE
C2 ONIRIS, FRANCE
   IFREMER, FRANCE
SI NANTES
SE PDG-RBE-BE
IN WOS Ifremer UPR
   copubli-france
IF 4.759
TC 8
UR https://archimer.ifremer.fr/doc/00604/71629/70076.pdf
LA English
DT Article
DE ;Light polycyclic aromatic hydrocarbons (PAHs);Dynamic HeadSpace (DHS);Thermo desorption (TDU);GC-MS/MS;Isotope dilution;Seafood
AB The aim of our work was to develop an analytical strategy to quantify naphthalene, acenaphthylene, acenaphthene, fluorene, phenanthrene and anthracene in fish products by on-line dynamic headspace extraction, followed by thermodesorption injection and gas chromatography analysis coupled with tandem mass spectrometry using electron ionization mode (DHS-TD-GC-EI-MS/MS). The developed protocol used 1 g of freeze-dried or oil sample supplemented with perdeuterated light PAHs. The sample was heated at [90 - 100°C], the headspace of the sample was swept by nitrogen and the trapping of the PAHs was carried out on a Tenax-type adsorbent placed at 25°C. Analytes were thermodesorbed at 300°C from the dried adsorbant and then cryofocused on a cooled injection system (CIS) at ¬25°C before injection (12°C s−1 up to 300°C). The chromatographic separation of PAHs was carried out on a 5-MS type column (30 m × 0.25 mm, 0.25 μm) and the acquisition of the signals was performed in SRM following the transitions, involving the loss of one or two hydrogen atoms from the molecular ion. In view of the principle of extraction, the calibration curve was performed on a representative matrix or using the standard addition method. Quantification limits were determined between 0.01 and 0.6 ng g−1 of matrix from the method blank results. The method was validated by a series of multi-level supplemented matrix assays and by the analysis of a reference material from an inter-laboratory test (mussels, IAEA-432). The average of the expanded measurement uncertainty was from 9 to 44% for the four lightest PAHs, except for fluorene when the sample incubation was set at 90°C. Occurrence measurements were performed on almost two hundred samples of molluscs, echinoderms and fish. The results have shown a quantification frequency greater than 66% for naphthalene and fluorene, at concentrations below a few ng g−1 of dry matter of fishery products. With this methodology, the light PAHs occurrence can now be measured in a wider range of foodstuffs in order to better characterize their contamination trends and the associated risk simultaneously.   
PY 2020
PD MAY
SO Journal Of Chromatography A
SN 0021-9673
PU Elsevier BV
VL 1619
UT 000530685300004
DI 10.1016/j.chroma.2020.460906
ID 71629
ER

EF