FN Archimer Export Format
PT J
TI Uptake of Inorganic and Organic Nitrogen Sources by Dinophysis acuminata and D. acuta
BT 
AF García-Portela, Maria
   Reguera, Beatriz
   Gago, Jesus
   LE GAC, Mickael
   Rodríguez, Francisco
AS 1:1;2:1;3:1;4:2;5:1;
FF 1:;2:;3:;4:PDG-ODE-DYNECO-PELAGOS;5:;
C1 Spanish Institute of Oceanography (IEO), Oceanographic Center of Vigo, Subida a Radio Faro 50, Cabo Estay, Canido, 36390 Vigo, Spain
   Ifremer, DYNECO PELAGOS, 29280 Plouzané, France
C2 IEO, SPAIN
   IFREMER, FRANCE
SI BREST
SE PDG-ODE-DYNECO-PELAGOS
IN WOS Ifremer UPR
   DOAJ
   copubli-europe
IF 4.167
TC 14
UR https://archimer.ifremer.fr/doc/00606/71770/70250.pdf
   https://archimer.ifremer.fr/doc/00606/71770/70251.pdf
LA English
DT Article
DE ;Dinophysis;nitrate;ammonium;urea;uptake rates;N-15 incubations;antibiotic treatment
AB Dinoflagellate species of Dinophysis are obligate mixotrophs that require light, nutrients, and prey for sustained growth. Information about their nitrogenous nutrient preferences and their uptake kinetics are scarce. This study aimed to determine the preferred nitrogen sources in cultures of D. acuminata and D. acuta strains from the Galician Rías Baixas (NW Spain) and to compare their uptake kinetics. Well-fed versus starved cultures of D. acuminata and D. acuta were supplied with N15 labeled inorganic (nitrate, ammonium) and organic (urea) nutrients. Both species showed a preference for ammonium and urea whereas uptake of nitrate was negligible. Uptake rates by well-fed cells of D. acuminata and D. acuta were 200% and 50% higher, respectively, than by starved cells. Uptake of urea by D. acuminata was significantly higher than that of ammonium in both nutritional conditions. In contrast, similar uptake rates of both compounds were observed in D. acuta. The apparent inability of Dinophysis to take up nitrate suggests the existence of incomplete nitrate-reducing and assimilatory pathways, in line with the paucity of nitrate transporter homologs in the D. acuminata reference transcriptome. Results derived from this study will contribute to understand Harmful Algal Blooms succession and differences in the spatio-temporal distribution of the two Dinophysis species when they co-occur in stratified scenarios. 
PY 2020
PD FEB
SO Microorganisms
SN 2076-2607
PU MDPI AG
VL 8
IS 2
UT 000519618200043
DI 10.3390/microorganisms8020187
ID 71770
ER

EF