FN Archimer Export Format PT J TI Biodiversity inventory of the grey mullets (Actinopterygii: Mugilidae) of the Indo‐Australian Archipelago through the iterative use of DNA‐based species delimitation and specimen assignment methods BT AF Delrieu‐Trottin, Erwan Durand, Jean‐Dominique Limmon, Gino Sukmono, Tedjo Sugeha, Hagi Yulia Chen, Wei‐Jen Busson, Frédéric Borsa, Philippe Dahruddin, Hadi Sauri, Sopian Fitriana, Yuli Zein, Mochamad Syamsul Arifin Hocdé, Régis Pouyaud, Laurent Keith, Philippe Wowor, Daisy Steinke, Dirk Hanner, Robert Hubert, Nicolas AS 1:1,2;2:3;3:4;4:5;5:6;6:7;7:8;8:1,9;9:10;10:1,11;11:11;12:11;13:11;14:3;15:1;16:9;17:11;18:12,13;19:12,13;20:1; FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;14:;15:;16:;17:;18:;19:;20:; C1 UMR 5554 ISEM (IRD, UM CNRS EPHE) Université de Montpellier Place Eugène Bataillon 34095 Montpellier cedex France Museum für Naturkunde Leibniz Institute for Evolution and Biodiversity Science Invalidenstr. 4310115 Berlin ,Germany UMR 9190 MARBEC (IRD, UM CNRS IFREMER) Université de Montpellier cc093, Place Eugène Bataillon34095Montpellier cedex ,France Universitas Pattimura Maritime and Marine Science Center of Excellence Jalan Wim Reawaru 9C678267 Ambon Moluccas, Indonesia Universitas Jambi Departement of Biology Jalan Lintas Jambi ‐ Muara Bulian Km15 36122 Jambi Sumatra, Indonesia Politeknik Kelautan dan Perikanan Sorong KKD BP. SR SGK Tanjung Kasuari Jl. Kapitan PattimuraKota Sorong 98401,Papua Barat , Indonesia Research Center for Oceanography Indonesian Institute of Sciences Jl Ancol Timur Pasir Putih 1Jakarta 14430, Indonesia Institute of Oceanography National Taiwan University Taipei , Taiwan UMR 7208 BOREA (MNHN CNRS UPMC IRD UCBN) Muséum National d’Histoire Naturelle 43 rue Cuvier 75231Paris cedex, France UMR 250 ENTROPIE (IRD, UR UNC CNRS IFREMER) centre IRD‐Occitanie 911 avenue Agropolis 34394 Montpellier ,France Division of Zoology Research Center for Biology Indonesian Institute of Sciences (LIPI) Jalan Raya Jakarta Bogor Km 46Cibinong 16911, Indonesia Centre for Biodiversity Genomics University of Guelph Ontario, Canada Department of Integrative Biology University of Guelph Guelph Ontario ,Canada C2 UNIV MONTPELLIER, FRANCE LEIBNIZ INST EVOL BIODIVERS SCI, GERMANY UNIV MONTPELLIER, FRANCE UNIV PATTIMURA, INDONESIA UNIV JAMBI, INDONESIA FISHERIES ACAD SORONG, INDONESIA INDONESIAN INST SCI, INDONESIA UNIV NATL TAIWAN NTU, TAIWAN MNHN, FRANCE IRD, FRANCE LIPI, INDONESIA UNIV GUELPH, CANADA UNIV GUELPH, CANADA UM MARBEC ENTROPIE IN WOS Cotutelle UMR DOAJ copubli-france copubli-europe copubli-int-hors-europe copubli-sud IF 5.183 TC 23 UR https://archimer.ifremer.fr/doc/00606/71810/70300.pdf https://archimer.ifremer.fr/doc/00606/71810/70301.pdf https://archimer.ifremer.fr/doc/00606/71810/70302.xlsx https://archimer.ifremer.fr/doc/00606/71810/70303.xlsx https://archimer.ifremer.fr/doc/00606/71810/70304.xlsx https://archimer.ifremer.fr/doc/00606/71810/70305.xlsx LA English DT Article DE ;Coral Triangle;cryptic diversity;DNA barcoding;reference library;taxonomic gap AB DNA barcoding opens new perspectives on the way we document biodiversity. Initially proposed to circumvent the limits of morphological characters to assign unknown individuals to known species, DNA barcoding has been used in a wide array of studies where collecting species identity constitutes a crucial step. The assignment of unknowns to knowns assumes that species are already well identified and delineated, making the assignment performed reliable. Here, we used DNA‐based species delimitation and specimen assignment methods iteratively to tackle the inventory of the Indo‐Australian Archipelago grey mullets, a notorious case of taxonomic complexity that requires DNA‐based identification methods considering that traditional morphological identifications are usually not repeatable and sequences mislabeling are common in international sequence repositories. We first revisited a DNA barcode reference library available at the global scale for Mugilidae through different DNA‐based species delimitation methods to produce a robust consensus scheme of species delineation. We then used this curated library to assign unknown specimens collected throughout the Indo‐Australian Archipelago to known species. A second iteration of OTUs delimitation and specimen assignment was then performed. We show the benefits of using species delimitation and specimen assignment methods iteratively to improve the accuracy of specimen identification and propose a workflow to do so. PY 2020 PD JUN SO Evolutionary Applications SN 1752-4571 PU Wiley VL 13 IS 6 UT 000512462700001 BP 1451 EP 1467 DI 10.1111/eva.12926 ID 71810 ER EF