Cryptic species in the parasitic Amoebophrya species complex revealed by a polyphasic approach

Type Article
Date 2020-02
Language English
Author(s) Cai Ruibo1, Kayal Ehsan2, Alves-De-Souza Catharina3, Bigeard Estelle1, Corre Erwan2, Jeanthon Christian1, Marie Dominique1, Porcel Betina M.4, Siano Raffaele5, Szymczak Jeremy1, Wolf Matthias6, Guillou Laure1
Affiliation(s) 1 : Sorbonne Université, CNRS, UMR7144 Adaptation et Diversité en Milieu Marin, Ecology of Marine Plankton (ECOMAP), Station Biologique de Roscoff SBR, 29680, Roscoff, France
2 : Sorbonne Université, CNRS, FR2424 ABIMS, Station Biologique de Roscoff SBR, 29680, Roscoff, France
3 : Algal Resources Collection, MARBIONC, Center for Marine Sciences, University of North Carolina Wilmington, 5600 Marvin K. Moss Lane, Wilmington, NC, 28409, US
4 : Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, University Evry, Université Paris-Saclay, 91057, Evry, France
5 : Ifremer-Centre de Bretagne, Département/Unité/Laboratoire ODE/DYNECO/Pelagos, Z.I. Technopôle Brest-Iroise, Pointe du Diable BP70, 29280, Plouzané, France
6 : Department of Bioinformatics, Biocenter, University of Würzburg, Am Hubland, 97074, Würzburg, Germany
Source Scientific Reports (2045-2322) (Springer Science and Business Media LLC), 2020-02 , Vol. 10 , N. 1 , P. 2531 (11p.)
DOI 10.1038/s41598-020-59524-z
WOS© Times Cited 4
Abstract

As critical primary producers and recyclers of organic matter, the diversity of marine protists has been extensively explored by high-throughput barcode sequencing. However, classification of short metabarcoding sequences into traditional taxonomic units is not trivial, especially for lineages mainly known by their genetic fingerprints. This is the case for the widespread Amoebophrya ceratii species complex, parasites of their dinoflagellate congeners. We used genetic and phenotypic characters, applied to 119 Amoebophrya individuals sampled from the same geographic area, to construct practical guidelines for species delineation that could be applied in DNA/RNA based diversity analyses. Based on the internal transcribed spacer (ITS) regions, ITS2 compensatory base changes (CBC) and genome k-mer comparisons, we unambiguously defined eight cryptic species among closely related ribotypes that differed by less than 97% sequence identity in their SSU rDNA. We then followed the genetic signatures of these parasitic species during a three-year survey of Alexandrium minutum blooms. We showed that these cryptic Amoebophrya species co-occurred and shared the same ecological niche. We also observed a maximal ecological fitness for parasites having narrow to intermediate host ranges, reflecting a high cost for infecting a broader host range. This study suggests that a complete taxonomic revision of these parasitic dinoflagellates is long overdue to understand their diversity and ecological role in the marine plankton.

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Cai Ruibo, Kayal Ehsan, Alves-De-Souza Catharina, Bigeard Estelle, Corre Erwan, Jeanthon Christian, Marie Dominique, Porcel Betina M., Siano Raffaele, Szymczak Jeremy, Wolf Matthias, Guillou Laure (2020). Cryptic species in the parasitic Amoebophrya species complex revealed by a polyphasic approach. Scientific Reports, 10(1), 2531 (11p.). Publisher's official version : https://doi.org/10.1038/s41598-020-59524-z , Open Access version : https://archimer.ifremer.fr/doc/00609/72156/