FN Archimer Export Format
PT J
TI Cryptic species in the parasitic Amoebophrya species complex revealed by a polyphasic approach
BT 
AF Cai, Ruibo
   Kayal, Ehsan
   Alves-de-Souza, Catharina
   Bigeard, Estelle
   Corre, Erwan
   Jeanthon, Christian
   Marie, Dominique
   Porcel, Betina M.
   Siano, Raffaele
   Szymczak, Jeremy
   Wolf, Matthias
   Guillou, Laure
AS 1:1;2:2;3:3;4:1;5:2;6:1;7:1;8:4;9:5;10:1;11:6;12:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:PDG-ODE-DYNECO-PELAGOS;10:;11:;12:;
C1 Sorbonne Université, CNRS, UMR7144 Adaptation et Diversité en Milieu Marin, Ecology of Marine Plankton (ECOMAP), Station Biologique de Roscoff SBR, 29680, Roscoff, France
   Sorbonne Université, CNRS, FR2424 ABIMS, Station Biologique de Roscoff SBR, 29680, Roscoff, France
   Algal Resources Collection, MARBIONC, Center for Marine Sciences, University of North Carolina Wilmington, 5600 Marvin K. Moss Lane, Wilmington, NC, 28409, US
   Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, University Evry, Université Paris-Saclay, 91057, Evry, France
   Ifremer-Centre de Bretagne, Département/Unité/Laboratoire ODE/DYNECO/Pelagos, Z.I. Technopôle Brest-Iroise, Pointe du Diable BP70, 29280, Plouzané, France
   Department of Bioinformatics, Biocenter, University of Würzburg, Am Hubland, 97074, Würzburg, Germany
C2 UNIV SORBONNE, FRANCE
   UNIV SORBONNE, FRANCE
   UNIV N CAROLINA, USA
   CEA, FRANCE
   IFREMER, FRANCE
   UNIV WURZBURG, GERMANY
SI BREST
SE PDG-ODE-DYNECO-PELAGOS
IN WOS Ifremer UPR
   DOAJ
   copubli-france
   copubli-europe
   copubli-univ-france
   copubli-int-hors-europe
IF 4.379
TC 19
UR https://archimer.ifremer.fr/doc/00609/72156/70916.pdf
   https://archimer.ifremer.fr/doc/00609/72156/70917.pdf
LA English
DT Article
AB As critical primary producers and recyclers of organic matter, the diversity of marine protists has been extensively explored by high-throughput barcode sequencing. However, classification of short metabarcoding sequences into traditional taxonomic units is not trivial, especially for lineages mainly known by their genetic fingerprints. This is the case for the widespread Amoebophrya ceratii species complex, parasites of their dinoflagellate congeners. We used genetic and phenotypic characters, applied to 119 Amoebophrya individuals sampled from the same geographic area, to construct practical guidelines for species delineation that could be applied in DNA/RNA based diversity analyses. Based on the internal transcribed spacer (ITS) regions, ITS2 compensatory base changes (CBC) and genome k-mer comparisons, we unambiguously defined eight cryptic species among closely related ribotypes that differed by less than 97% sequence identity in their SSU rDNA. We then followed the genetic signatures of these parasitic species during a three-year survey of Alexandrium minutum blooms. We showed that these cryptic Amoebophrya species co-occurred and shared the same ecological niche. We also observed a maximal ecological fitness for parasites having narrow to intermediate host ranges, reflecting a high cost for infecting a broader host range. This study suggests that a complete taxonomic revision of these parasitic dinoflagellates is long overdue to understand their diversity and ecological role in the marine plankton. 
PY 2020
PD FEB
SO Scientific Reports
SN 2045-2322
PU Springer Science and Business Media LLC
VL 10
IS 1
UT 000562888500007
DI 10.1038/s41598-020-59524-z
ID 72156
ER

EF