FN Archimer Export Format
PT J
TI A proteomic study of resistance to Brown Ring disease in the Manila clam, Ruditapes philippinarum
BT 
AF Smits, M.
   Artigaud, Sebastien
   Bernay, B.
   Pichereau, Vianney
   Bargelloni, L.
   Paillard, Christine
AS 1:1,2;2:1;3:3;4:1;5:2;6:1;
FF 1:;2:;3:;4:;5:;6:;
C1 Université de Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280, Plouzané, France
   Department of Comparative Biomedicine and Food Science, University of Padova, Agripolis Campus, Viale dell'Universita', 16, 35020, Legnaro (PD), Italy
   Plateforme Proteogen, SFR ICORE 4206, Université de Caen Basse-Normandie, Esplanade de la paix, 14032, Caen cedex, France
C2 UBO, FRANCE
   UNIV PADOVA, ITALY
   UNIV CAEN BASSE NORMANDIE, FRANCE
UM LEMAR
IN WOS Cotutelle UMR
   copubli-france
   copubli-europe
   copubli-univ-france
IF 4.581
TC 13
UR https://archimer.ifremer.fr/doc/00615/72670/71758.pdf
LA English
DT Article
DE ;Bivalve;Diseases;Resistance;Manila clam;Vibrio tapetis;Proteomics;Immune response
AB Marine mollusk aquaculture has more than doubled over the past twenty years, accounting for over 15% of total aquaculture production in 2016. Infectious disease is one of the main limiting factors to the development of mollusk aquaculture, and the difficulties inherent to combating pathogens through antibiotic therapies or disinfection have led to extensive research on host defense mechanisms and host-pathogen relationships. It has become increasingly clear that characterizing the functional profiles of response to a disease is an essential step in understanding resistance mechanisms and moving towards more effective disease control. The Manila clam, Ruditapes philippinarum, is a main cultured bivalve species of economic importance which is affected by Brown Ring disease (BRD), an infection induced by the bacterium Vibrio tapetis. In this study, juvenile Manila clams were subjected to a 28-day controlled challenge with Vibrio tapetis, and visual and molecular diagnoses were carried out to distinguish two extreme phenotypes within the experimental clams: uninfected (“RES”, resistant) and infected (“DIS”, diseased) post-challenge. Total protein extractions were carried out for resistant and diseased clams, and proteins were identified using LC-MS/MS. Protein sequences were matched against a reference transcriptome of the Manila clam, and protein intensities based on label-free quantification were compared to reveal 49 significantly accumulated proteins in resistant and diseased clams. Proteins with known roles in pathogen recognition, lysosome trafficking, and various aspects of the energy metabolism were more abundant in diseased clams, whereas those with roles in redox homeostasis and protein recycling were more abundant in resistant clams. Overall, the comparison of the proteomic profiles of resistant and diseased clams after a month-long controlled challenge to induce the onset of Brown Ring disease suggests that redox homeostasis and maintenance of protein structure by chaperone proteins may play important and interrelated roles in resistance to infection by Vibrio tapetis in the Manila clam. 
PY 2020
PD APR
SO Fish & Shellfish Immunology
SN 1050-4648
PU Elsevier BV
VL 99
UT 000523601500066
BP 641
EP 653
DI 10.1016/j.fsi.2020.02.002
ID 72670
ER

EF