Diversity of quorum sensing autoinducer synthases in the Global Ocean Sampling metagenomic database

Type Article
Date 2015
Language English
Author(s) Doberva Margot1, Sanchez-Ferandin Sophie2, Toulza Eve3, 4, Lebaron Philippe1, Lami Raphael1
Affiliation(s) 1 : Univ Paris 06, Sorbonne Univ, Observ Oceanol, CNRS,LBBM, F-66650 Banyuls Sur Mer, France.
2 : Univ Paris 06, Sorbonne Univ, Observ Oceanol, CNRS,BIOM, F-66650 Banyuls Sur Mer, France.
3 : UPVD Univ Perpignan, UMR 5244, IHPE, F-66860 Perpignan 9, France.
4 : CNRS, UMR 5244, 2EI, F-66860 Perpignan 9, France.
Source Aquatic Microbial Ecology (0948-3055) (Inter-research), 2015 , Vol. 74 , N. 2 , P. 107-119
DOI 10.3354/ame01734
WOS© Times Cited 38
Keyword(s) Quorum sensing, Acyl-homoserine lactone synthases, Global Ocean Sampling, Environmental metagenomes

Quorum sensing (QS) is a cell-to-cell signalling pathway that allows bacteria to synchronize their genetic expression. It is mediated by autoinducers (AI), including (1) acyl-homoserine lactones (AHLs or AI-1), produced by Proteobacteria using AinS, LuxI and HdtS synthase families and (2) furanosyl-diester-borate (FDB or AI-2), produced by a large range of phylogenetically diverse bacteria and synthetized by the LuxS family. Few data have been collected about the presence and importance of QS in marine waters using culture independent methods. In this study, we examined the presence and the diversity of AI-1 and AI-2 synthases in the Global Ocean Sampling (GOS), a large metagenomic database, covering 68 stations across 3 oceans. We built 4 reference protein databases with maximal phylogenetic coverage containing all known AI synthase sequences to retrieve AI synthases sequences from the GOS metagenomes. We retrieved 29 environmental sequences affiliated to LuxI (synthesizing AI-1), 653 related to HdtS (AI-1), 31 related to LuxS (AI-2) and only one for AinS (AI-1). AI synthases sequences were found in the 3 oceans covered by the GOS cruise and spanned a large phylogenetic diversity. These data revealed a large number of new marine AI sequences, suggesting that QS based on AI-1 diffusion is a widespread mechanism in the marine environment.

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Doberva Margot, Sanchez-Ferandin Sophie, Toulza Eve, Lebaron Philippe, Lami Raphael (2015). Diversity of quorum sensing autoinducer synthases in the Global Ocean Sampling metagenomic database. Aquatic Microbial Ecology, 74(2), 107-119. Publisher's official version : https://doi.org/10.3354/ame01734 , Open Access version : https://archimer.ifremer.fr/doc/00615/72718/