FN Archimer Export Format PT J TI Molecular characterisation of immunological memory following homologous or heterologous challenges in the schistosomiasis vector snail, Biomphalaria glabrata BT AF PINAUD, Silvain PORTET, Anais ALLIENNE, Jean-Francois BELMUDES, Lucid SAINT-BEAT, Cecile ARANCIBIA, Nathalie GALINIER, Richard DU PASQUIER, Louis DUVAL, David GOURBAL, Benjamin AS 1:1,4;2:1;3:1;4:2;5:1;6:1;7:1;8:3;9:1;10:1; FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:; C1 Univ Montpellier, Univ Perpignan, Interact Hates Pathogens Environm, UMR 5244,CNRS, Via Domitia, F-66860 Perpignan, France. CEA Grenoble Exploring Dynam Proteomes EDyP, F-38054 Grenoble 9, France. Univ Basel, Zool Inst, Dept Zool & Evolutionary Biol, Vesalgasse 1, Basel, Switzerland. Wellcome Trust Sanger Inst, Hinxton CB10 1SA, England. C2 UNIV MONTPELLIER, FRANCE CEA, FRANCE UNIV BASEL, SWITZERLAND WELLCOME TRUST SANGER INST, UK UM IHPE IN WOS Cotutelle UMR copubli-france copubli-europe copubli-int-hors-europe IF 3.192 TC 19 UR https://archimer.ifremer.fr/doc/00615/72738/87074.pdf LA English DT Article DE ;Immunological memory;Specificity;Schistosoma;Biomphalaria;RNAseq;Proteomic;Vaccination AB Invertebrate immune response may be primed by a current infection in a sustained manner, leading to the failure of a secondary infection with the same pathogen. The present study focuses on the Schistosomiasis vector snail Biomphalaria glabrata, in which a specific genotype-dependent immunological memory was demonstrated as a shift from a cellular to a humoral immune response. Herein, we investigate the complex molecular bases associated with this genotype-dependant immunological memory response. We demonstrate that Biomphalaria regulates a polymorphic set of immune recognition molecules and immune effector repertoires to respond to different strains of Schistosoma parasites. These results suggest a combinatorial usage of pathogen recognition receptors (PRRs) that distinguish different strains of parasites during the acquisition of immunological memory. Immunizations also show that snails become resistant after exposure to parasite extracts. Hemolymph transfer and a label-free proteomic analysis proved that circulating hemolymph compounds can be produced and released to more efficiently kill the newly encountered parasite of the same genetic lineage. PY 2019 PD MAR SO Developmental And Comparative Immunology SN 0145-305X PU Elsevier Sci Ltd VL 92 UT 000458596200025 BP 238 EP 252 DI 10.1016/j.dci.2018.12.001 ID 72738 ER EF