FN Archimer Export Format
PT J
TI Novel Antifungal Compounds, Spermine-Like and Short Cyclic Polylactates, Produced by Lactobacillus harbinensis K.V9.3.1Np in Yogurt
BT 
AF MOSBAH, Amor
   DELAVENNE, Emilie
   SOUISSI, Yasmine
   MAHJOUBI, Mouna
   JEHAN, Philippe
   LE YONDRE, Nicolas
   CHERIF, Ameur
   BONDON, Arnaud
   MOUNIER, Jerome
   BAUDY-FLOC'H, Michele
   LE BLAY, Gwenaelle
AS 1:1,2;2:3,4;3:2;4:2;5:5;6:5;7:2;8:1;9:3,4;10:1;11:3,4,6;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;
C1 Univ Rennes 1, CNRS, ISCR UMR 6226, Rennes, France.
   Univ Manouba, Biotechpole Sidi Thabet, ISBST, BVBGR LR11ES31, Ariana, Tunisia.
   Technopole Brest Iroise, ESIAB, ScInBioS SFR148, UEB,EA3882, Plouzane, France.
   Univ Brest, Lab Univ Biodiversite & Ecol Microbienne, Plouzane, France.
   Univ Rennes, CNRS, ScanMAT UMS 2001, Rennes, France.
   Univ Europeenne Bretagne, Univ Brest, Lab Sci Environm Marin, Inst Univ Europeen Mer,UMR 6539, Plouzane, France.
C2 UNIV RENNES, FRANCE
   UNIV MANOUBA, TUNISIA
   ESIAB, FRANCE
   UBO, FRANCE
   UNIV RENNES, FRANCE
   UBO, FRANCE
UM LEMAR
IN DOAJ
IF 4.259
TC 13
UR https://archimer.ifremer.fr/doc/00616/72773/72221.pdf
LA English
DT Article
DE ;spermine;polylactates;lactobacillus;extraction;purification;Ms;Nmr
AB Lactobacillus harbinensis K.V9.3.1Np was described as endowed with high antifungal activity. Most of the studies associated this activity to the produced organic acids, i.e., lactic acid, acetic acid, and hexanoic acid. The aim of this study was to purify and identify, other not yet described, antifungal molecules produced by L. harbinensis K.V9.3.1 Np when used in yogurt fermentation. Active compounds were extracted through several extraction processes using organic solvents and protein precipitation. The fractions of interest were purified using flash chromatography and preparative HPLC for specific characterization. The bioactive compounds identification was performed using Nuclear Magnetic Resonance and Mass Spectrometry. Activity tests against Penicillium expansum and Yarrowia lipolytica showed that the active compounds from L. harbinensis K.V9.3.1Np are benzoic acid and a polyamine identified as a spermine analog, which has not been reported earlier. However, the highest activity was shown by a mixture of short (n = 2-5) polycyclic lactates. Our overall results demonstrate the efficiency of the proposed extraction/purification approach. The new compounds described here have promising antifungal activities but further studies are still needed to decipher their mode of action and production pathways. Even though, they present an interesting potential application in food, feed, as well as, in pharmaceutical industries and could serve as alternative to chemical additives. 
PY 2018
PD OCT
SO Frontiers In Microbiology
SN 1664-302X
PU Frontiers Media Sa
VL 9
UT 000446713600001
DI 10.3389/fmicb.2018.02252
ID 72773
ER

EF