Carbon partitioning and lipid remodeling during phosphorus and nitrogen starvation in the marine microalga Diacronema lutheri (Haptophyta)

Type Article
Date 2020-08
Language English
Author(s) Huang Bing1, Mimouni Virginie2, Lukomska Ewa3, Morant-Manceau Annick1, Bougaran GaelORCID3
Affiliation(s) 1 : Laboratoire Mer, Molécules; Santé (IUML - FR 3473 CNRS), UFR Sciences et Techniques, Le Mans Université, avenue Olivier Messiaen; 72085 Le Mans Cedex, France
2 : Laboratoire Mer, Molécules; Santé (IUML - FR 3473 CNRS), IUT de Laval, Département Génie Biologique, Le Mans Université; 52 rue des Docteurs Calmette et Guérin 53020 Laval Cedex, France
3 : Laboratoire Physiologie et Biotechnologie des Algues; IFREMER; rue de l'Ile d'Yeu, BP 21105 44311 Nantes Cedex, France
Source Journal Of Phycology (0022-3646) (Wiley), 2020-08 , Vol. 56 , N. 4 , P. 908-922
DOI 10.1111/jpy.12995
WOS© Times Cited 2
Keyword(s) carbon partitioning, Diacronema lutheri, nitrogen, phosphorus, polyunsaturated fatty acid
Abstract

The domesticated marine microalga Diacronema lutheri is of great interest for producing various highly valuable molecules like lipids, particularly long‐chain polyunsaturated fatty acids (LC‐PUFA). In this study, we investigated the impact of phosphorus (P) and nitrogen (N) starvation on growth, carbon fixation (photosynthetic activity) and partitioning, and membrane lipid remodeling in this alga during batch culture. Our results show that the photosynthetic machinery was similarly affected by P and N stress. Under N starvation, we observed a much lower photosynthetic rate and biomass productivity. The degradation and re‐use of cellular N‐containing compounds contributed to triacylglycerol (TAG) accumulation. On the other hand, P‐starved cells maintained pigment content and a carbon partitioning pattern more similar to the control, ensuring a high biomass. Betaine lipids constitute the major compounds of non‐plastidial membranes, which are rich in eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids. Under P and N starvations, EPA was transferred from the recycling of membrane polar lipids, most likely contributing to TAG accumulation.

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Huang Bing, Mimouni Virginie, Lukomska Ewa, Morant-Manceau Annick, Bougaran Gael (2020). Carbon partitioning and lipid remodeling during phosphorus and nitrogen starvation in the marine microalga Diacronema lutheri (Haptophyta). Journal Of Phycology, 56(4), 908-922. Publisher's official version : https://doi.org/10.1111/jpy.12995 , Open Access version : https://archimer.ifremer.fr/doc/00619/73071/