TY - JOUR T1 - Desulfobotulus mexicanus sp. nov., a novel sulfate-reducing bacterium isolated from the sediment of an alkaline crater lake in Mexico A1 - Pérez-Bernal,Maria Fernanda A1 - Brito,Elcia M. S. A1 - Bartoli,Manon A1 - Aubé,Johanne A1 - Ollivier,Bernard A1 - Guyoneaud,Rémy A1 - Hirschler-Réa,Agnès AD - Aix Marseille Univ., Université de Toulon, CNRS, IRD, MIO UM 110, 13288, Marseille, France AD - Laboratory of Sanitary and Environmental Engineering, Engineering Division, Campus de Guanajuato, University of Guanajuato, Guanajuato, Mexico AD - Environmental Microbiology group, IPREM UMR CNRS 5254, Université de Pau et des Pays de l'Adour, IBEAS, Pau, France UR - https://archimer.ifremer.fr/doc/00622/73396/ DO - 10.1099/ijsem.0.004159 KW - alkaliphilic anaerobic bacterium KW - sulfate reduction KW - Desulfobotulus N2 - A novel Gram-negative, non-spore-forming, vibrio-shaped, anaerobic, alkaliphilic, sulfate-reducing bacterium, designated strain PAR22NT, was isolated from sediment samples collected at an alkaline crater lake in Guanajuato (Mexico). Strain PAR22NT grew at temperatures between 15 and 37 °C (optimum, 32 °C), at pH between pH 8.3 and 10.1 (optimum, pH 9.0–9.6), and in the presence of NaCl up to 10 %. Pyruvate, 2-methylbutyrate and fatty acids (4–18 carbon atoms) were used as electron donors in the presence of sulfate as a terminal electron acceptor and were incompletely oxidized to acetate and CO2. Besides sulfate, both sulfite and elemental sulfur were also used as terminal electron acceptors and were reduced to sulfide. The predominant fatty acids were summed feature 10 (C18 : 1 ω7c and/or C18 : 1 ω9t and/or C18 : 1 ω12t), C18 : 1 ω9c and C16 : 0. The genome size of strain PAR22NT was 3.8 Mb including 3391 predicted genes. The genomic DNA G+C content was 49.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that it belongs to the genus Desulfobotulus within the class Deltaproteobacteria. Its closest phylogenetic relatives are Desulfobotulus alkaliphilus (98.4 % similarity) and Desulfobotulus sapovorans (97.9 % similarity). Based on phylogenetic, phenotypic and chemotaxonomic characteristics, we propose that the isolate represents a novel species of the genus Desulfobotulus with the name Desulfobotulus mexicanus sp. nov. The type strain is PAR22NT (=DSM 105758T=JCM 32146T). Y1 - 2020 PB - Microbiology Society JF - International Journal Of Systematic And Evolutionary Microbiology SN - 1466-5026 VL - 70 IS - 5 SP - 3219 EP - 3225 ID - 73396 ER -